[关键词]
[摘要]
目的 对同株不同生长年限的白芍Paeonia lactiflora化学成分差异进行比较研究,为白芍药材最佳采收和质量控制提供实验数据支持。方法 基于HPLC方法建立的白芍指纹图谱,应用于同株不同生长年限白芍,并对其相似性和差异性进行分析,在此基础上对其中没食子酸、氧化芍药苷、芍药内酯苷、芍药苷、苯甲酸、1,2,3,4,6-O-五没食子酰葡萄糖、苯甲酰芍药苷7种成分进行含量测定。利用中药色谱指纹图谱相似度评价系统(2012A版)计算相似度,运用主成分分析(PCA)和聚类分析对样品进行模式识别研究。结果 同株不同生长年限的白芍指纹图谱相似性较高,特征峰差异性体现于有4个峰在低年限中存在,另有1个峰只在高年限中存在。有5个峰存在增加趋势,15个峰存在降低趋势。白芍生长过程中芍药内酯苷呈现降低趋势,芍药苷、1,2,3,4,6-O-五没食子酰葡萄糖呈现增加趋势。白芍随着年限的增加,其最高年限的比值始终最大,且其干质量比例增加。相似度结果显示白芍不同年限间表现出较强的一致性,相邻年限间相似性更大,聚类分析发现,白芍随着生长年限增加,含量逐渐稳定。另采用综合质量数对不同年限白芍进行评价,结果显示白芍5年生采收最佳。结论 同株不同生长年限白芍化学成分和主要成分含量上存在一定差异,建议在第5年采收。
[Key word]
[Abstract]
Objective To compare and study the chemical composition differences of Paeoniae Alba Radix with different growth, in order to provide experimental data support for the best collection and quality control of Paeoniae Alba Radix. Methods Based on HPLC method, the fingerprint of Paeoniae Alba Radix was applied to different growth years of the same plant, and its similarity and difference were analyzed. On this basis, the contents of seven components were determined. The similarity was calculated by the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine (2012A version), and the pattern recognition of the samples was studied by principal component analysis (PCA) and cluster analysis. Results The fingerprint of Paeoniae Alba Radix with different growth years of the same plant was similar, and the difference of characteristic peaks was reflected in the existence of four peaks in the low years. One peak only existed in the high years. There was an increasing trend in five peaks and a decreasing trend in 15 peaks. During the growth of Paeoniae Alba Radix in Bozhou, alibiflorin showed a decreasing trend, while paeoniflorin and 1,2,3,4,6-pentagalloyglucose showed an increasing trend during the growth of Paeoniae Alba Radix. With the increase of years, the ratio of the highest years of Paeoniae Alba Radix was always the largest, and the proportion of dry weight of Paeoniae Alba Radix increased. The similarity results showed that there was a strong consistency among different years of Paeoniae Alba Radix, and the similarity between adjacent years was greater. Cluster analysis showed that the content of Paeonia lactiflora was stable with the increase of growth years. In addition, the comprehensive mass number was used to evaluate Paeoniae alba Radix in different years. The results showed that the best harvest time of Paeoniae Alba Radix was in the fifth year. Conclusion The chemical components and main components of Paeoniae Alba Radix with different growth years were different. It is suggested that Paeoniae Alba Radix should be collected in the fifth year.
[中图分类号]
R286.2
[基金项目]
国家重点研发计划资助项目(2017YFC1701600)