目的 探讨延胡索、白芷炮制配伍对元胡止痛方中延胡索乙素、欧前胡素含量以及镇痛作用的影响。方法 延胡索、白芷按不同比例炮制配伍，比较单味药及其不同配伍复方中延胡索乙素、欧前胡素含量，比较单味药及其不同配伍复方对小鼠醋酸扭体反应、小鼠热板法镇痛和缩宫素诱发大鼠痛经模型的影响。结果 延胡索经醋炙后延胡索乙素质量分数增加，配伍复方中延胡索乙素质量分数有升有降，以醋炙延胡索-白芷（2∶1）配伍复方中质量分数最高；欧前胡素在白芷中质量分数最高，在配伍过程中降低；延胡索、白芷及其炮制配伍复方对小鼠、大鼠均有明显镇痛作用（P<0.05、0.01），配伍复方的镇痛作用优于单味药，其中以醋炙延胡索-白芷（2∶1）配伍复方的镇痛作用最强。结论 延胡索经醋炙后镇痛作用增强，延胡索、白芷在炮制配伍过程中产生协同镇痛作用，以醋炙延胡索-白芷（2∶1）配伍复方镇痛作用最佳。

Objective To explore the effect of processing and compatibility of Yanhusuo (Corydalis Rhizoma) and Baizhi (Angelicae Dahuricae Radix) on contents of tetrahydropalmatine and imperatorin in Yuanhu Zhitong Recipe and its analgesic effect. Methods Compatibility of Corydalis Rhizoma and Angelicae Dahuricae Radix were processed in different proportions, contents of tetrahydropalmatine and imperatorin in single medicine and different compatibility compounds were compared. Effects of single medicine and different compatibility compounds on writhing reaction induced by acetic acid in mice, pain threshold induced by hot plate method in mice and oxytocin-induced writhing response in dysmenorrhea models of rats were compared. Results The content of tetrahydropalmatine was increased after Corydalis Rhizoma being vinegar-roasted, and content of tetrahydropalmatine was increased and decreased in compatibility compound, content of tetrahydropalmatine in vinegar-roasted Corydalis Rhizoma-Angelicae Dahuricae Radix (2:1) compound was the highest. The content of imperatorin in Angelicae Dahuricae Radix was the highest, and was decreased in process of compatibility. Single drugs and processing and compatibility of Corydalis Rhizoma and Angelicae Dahuricae Radix had significant analgesic effects on mice and rats, and the analgesic effect of combined compound was better than that of single drugs. The analgesic effect of vinegar-roasted Corydalis Rhizoma-Angelicae Dahuricae Radix (2:1) was the strongest. Conclusion The analgesic effect of Corydalis Rhizoma is enhanced after vinegar roasting, Corydalis Rhizoma and Angelicae Dahuricae Radix have synergistic analgesic effects in processing and compatibility, and the analgesic effect of vinegar-roasted Corydalis Rhizoma-Angelicae Dahuricae Radix (2:1) is the best.

R285.5

国家自然科学基金资助项目（81803594）