目的 建立一测多评法（quantitative analysis single-marker，QAMS）测定红参药材中11种皂苷类成分的含量，为红参质量控制提供科学依据。方法 采用HPLC法，Agilent C₁₈色谱柱（250 mm×4.6 mm，5μm），以乙腈-0.1%磷酸水溶液为流动相梯度洗脱，体积流量1.3 mL/min，柱温30℃，波长203 nm。以人参皂苷Rb₁为参照物，建立其与人参皂苷Rg₁、人参皂苷Re、人参皂苷Rf、人参皂苷Rh₁、人参皂苷Rc、人参皂苷Ro、人参皂苷Rb₂、人参皂苷Rb₃、人参皂苷Rd、人参皂苷Rg₃的相对校正因子，外标法与QAMS分别测定11个成分含量。结果 在一定线性范围内，人参皂苷Rb₁相对人参皂苷Rg₁、人参皂苷Re、人参皂苷Rf、人参皂苷Rh₁、人参皂苷Rc、人参皂苷Ro、人参皂苷Rb₂、人参皂苷Rb₃、人参皂苷Rd、人参皂苷Rg₃的相对校正因子重复性好，2种方法所得11种成分的含量无明显差异，实验得相对校正因子可供参考。结论 本研究建立的QAMS法，快速、简便、重复性好，可为红参药材的质量控制提供参考。

Objective To establish a quantitative analysis of multi-components by single maker (QAMS) for the content determination of eleven saponins in red ginseng, so as to provide a scientific basis for the quality control of red ginseng,. Methods The HPLC analysis was performed on Agilent C₁₈column (250 mm×4.6 mm, 5 μm), using acetonitrile and 0.1% phosphoric acid solution as the mobile phase at a flow rate of 1.3 mL/min, and column temperature was 30℃ and the detection wavelength was set at 203 nm. Ginsenoside Rb₁ was used as reference to establish its relative correction factor of Rg₁, Re, Rf, Rh₁, Rc, Ro, Rb₂, Rb₃, Rd and Rg₃. The contents of eleven components were determined by both external standard method and QAMS. Results Within a certain linear range, ginsenoside Rb₁ had better reproducibility than ginsenoside Rg₁, Re, Rf, Rh₁, Rc, Ro, Rb₂, Rb₃, Rd, and Rg₃ in terms of relative correction factors. There was no significant difference in the contents of 11 components obtained by the two methods. The relative correction factors obtained in the experiment could be used for reference. Conclusion The establishment of multi-components by single maker method in this study is rapid, simple and reproducible, which can provide reference for the quality control of red ginseng.

R286

华北理工大学博士科研启动基金项目（No.28418499）