目的 利用斑马鱼模型和中医药整合药理学平台（TCMIP），探究天麻抗癫痫的主要活性成分及潜在的作用机制。方法 采用健康发育至72 h的AB系斑马鱼幼鱼为实验动物，以氨茶碱诱导形成癫痫模型，观察天麻对斑马鱼癫痫样行为的改善作用；利用TCMIP筛选天麻抗癫痫的靶点，构建天麻与癫痫的蛋白质相互作用（protein-protein interaction，PPI）网络，绘制药材-成分-靶点-通路-疾病网络；利用Autodock vina软件对活性成分和靶点进行分子对接。结果 天麻对氨茶碱诱导的斑马鱼的癫痫样行为发生频率具有显著改善作用（P<0.05）。通过TCMIP v2.0整合分析发现，天麻抗癫痫的核心靶点有25个，天麻素、天麻醚苷、谷甾醇、棕榈酸、西托糖苷可能是天麻抗癫痫的主要活性成分；这些活性成分可以直接作用于相应关键靶点，参与调节神经递质的平衡、维持神经元细胞正常电生理活动并影响酮体生成。结论 天麻对癫痫样症状具有明显的改善作用，其抗癫痫的潜在机制可能与调节神经递质的平衡、神经元细胞的电生理稳态和酮体的生成有关，为天麻的临床应用提供理论依据。

Objective To explore the key components and potential mechanism of antiepileptic effect of Tianma (Gastrodiae Rhizoma) by using zebrafish model and TCMIP. Methods AB zebrafish larva that have grown healthily to 72 h are used as experimental animals. They were treated by aminophylline to establish epileptic models to observe the effect of Gastrodiae Rhizoma on the improvement of zebrafish's epileptic like behavior. TCMIP was used to screen the candidate anti-epilepsy targets, the protein-protein interaction network (PPI) of Gastrodiae Rhizoma with disease was constructed, and the network map of Chinese medicinal herbs-components-core targets-pathways-diseases was drawn. Autodock Vina software was used to dock small molecules in Gastrodiae Rhizoma with proteins. Results Gastrodiae Rhizoma had a protective effect on aminophylline-induced epileptic model of zebrafish (P < 0.05). The integrated analysis based on TCMIP v2.0 found that there were 25 core targets for anti-epilepsy of Gastrodiae Rhizoma, and five key pharmacodynamic compounds included gastrodin, gastrodioside, sitosterol, palmitic acid and sitogluside might be response to the anti-epilepsy effect of Gastrodiae Rhizoma. These compounds could directly act on the corresponding core target proteins, participate in the balance of neurotransmitter, maintain normal electrophysiological activities of neuron cells and affect ketone bodies formation. Conclusion Gastrodiae Rhizoma could significantly improve epileptiform symptoms on zebrafish model. The potential mechanism of network regulation about anti-epileptic may be related to the balance of neurotransmitters, the electrophysiological homeostasis of neuronal cells and the production of ketone bodies, which provides theoretical basis for clinical application of Gastrodiae Rhizoma.

R285.5

山东省自然科学基金资助项目（ZR2019MH037）；山东省园区产业提升工程项目（2019YQ033）；山东第一医科大学学术提升计划（2019LJ003）