目的 研究附子-干姜对慢性溃疡性结肠炎（ulcerative colitis，UC）小鼠肠黏膜微循环障碍的改善作用。方法 建立慢性UC小鼠模型，给予附子-干姜水煎液进行干预，记录各组小鼠体质量、大便性状及便血情况，进行疾病活动指数（disease activity index，DAI）评分；采用激光多普勒血流仪检测各组小鼠肠黏膜血流灌注量、血细胞移动速度和移动血细胞浓度；采用ELISA法检测各组小鼠血清中血小板活化因子（platelet activating factor，PAF）和组织因子（tissue factor，TF）水平；采用苏木素-伊红（HE）染色法观察各组小鼠结肠组织病理改变情况；采用免疫组化法检测各组小鼠肠黏膜毛细血管内皮细胞CD31表达并计算微血管密度（microvessel density，MVD）。结果 与对照组比较，模型组小鼠DAI评分显著增加（P<0.01），肠黏膜血流灌注量、血细胞移动速度显著下降（P<0.01），肠黏膜移动血细胞浓度显著升高（P<0.01），血清PAF、TF水平显著升高（P<0.01），结肠组织CD31表达减少，肠黏膜微血管密度显著减少（P<0.01）；与模型组比较，附子-干姜高剂量（6.06 g/kg）组小鼠DAI评分明显降低（P<0.01），肠黏膜血流灌注量、血细胞移动速度显著升高（P<0.01），肠黏膜移动血细胞浓度显著降低（P<0.01），机体凝血因子PAF、TF水平显著降低（P<0.01），肠黏膜MVD显著增加（P<0.01）。结论 附子-干姜可通过改善肠黏膜微循环障碍从而治疗慢性UC。

Objective To study the effect of Fuzi (Aconiti Lateralis Radix Praeparaia)-Ganjiang (Zingiberis Rhizoma) on intestinal mucosal microcirculation disorder in mice with chronic ulcerative colitis (UC). Methods Mice model of chronic UC was established, and Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma decoction was given to intervene. Body weight, stool characteristics and blood in stool were recorded, disease activity index (DAI) score was performed. Laser Doppler Blood Flow Meter was used to detect intestinal mucosal blood perfusion, blood cell movement speed and mobile blood cell concentration. ELISA method was used to detect platelet activating factor (PAF) and tissue factor (TF) levels in serum. Pathological changes of colon tissue were observed by hematoxylin-eosin (HE) staining. Immunohistochemical method was used to detect the expression of CD31 in intestinal mucosal capillary endothelial cells of mice and the microvessel density (MVD) was calculated. Results Compared with control group, DAI score of mice in model group was significantly increased (P < 0.01), intestinal mucosal blood perfusion and blood cell moving speed were significantly decreased (P < 0.01), the concentration of moving blood cells in intestinal mucosa was significantly increased (P < 0.01), PAF and TF levels in serum were significantly increased (P < 0.01), CD31 expression in colon tissue was reduced, and intestinal mucosal microvessel density was significantly reduced (P < 0.01). Compared with model group, DAI score of mice in high-dose (6.06 g/kg) Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma group was significantly reduced (P < 0.01), intestinal mucosal blood perfusion and blood cell moving speed were significantly increased (P < 0.01), concentration of moving blood cells in intestinal mucosa was significantly reduced (P < 0.01), and coagulation factor PAF and TF levels in serum were significantly reduced (P < 0.01), MVD of intestinal mucosa was significantly increased (P < 0.01). Conclusion Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma can treat chronic UC by improving intestinal mucosal microcirculation.

R285.5

国家自然科学基金地区基金资助项目（81860728）