[关键词]
[摘要]
目的 研究重楼皂苷Ⅶ对人胰腺癌PANC-1细胞增殖、迁移与侵袭作用及机制。方法 采用MTT法检测重楼皂苷Ⅶ对PANC-1细胞存活率的影响；观察重楼皂苷Ⅶ对PANC-1细胞形态的影响；采用划痕实验和Transwell小室法考察重楼皂苷Ⅶ对PANC-1细胞迁移和侵袭能力的影响；采用流式细胞术检测重楼皂苷Ⅶ对PANC-1细胞凋亡的影响；采用Western blotting法检测重楼皂苷Ⅶ对PANC-1细胞半胱氨酸蛋白酶-3（Caspase-3）、B细胞淋巴瘤-2（B-cell lymphoma-2，Bcl-2）、Bcl-2相关X蛋白（Bcl-associated X protein，Bax）、聚腺苷二磷酸核糖聚合酶（poly ADP-ribose polymerase，PARP）、DNA酶抑制物（inhibitor of caspase-activated deoxyribonuclease，ICAD）和程序性死亡分子配体-1（programmed death ligand 1，PD-L1）蛋白表达的影响。结果 重楼皂苷Ⅶ可抑制PANC-1细胞存活率，显著抑制PANC-1细胞迁移和侵袭能力（P<0.01），显著诱导PANC-1细胞凋亡（P<0.05、0.01），显著升高PANC-1细胞中凋亡相关蛋白Cleaved Caspase-3、PARP、Bax表达水平（P<0.01），显著降低pro-PARP、ICAD、Bcl-2、PD-L1蛋白表达水平（P<0.05、0.01）。结论 重楼皂苷Ⅶ能够抑制PANC-1细胞增殖、迁移和侵袭，并可能通过下调PD-L1表达诱导PANC-1细胞凋亡。
[Key word]
[Abstract]
Objective To study the effect and mechanism of polyphyllin Ⅶ on proliferation, migration and invasion of human pancreatic cancer PANC-1 cells. Methods MTT assay was used to detect the effect of polyphyllin Ⅶ on viability of PANC-1 cells. Effect of polyphyllin Ⅶ on morphology of PANC-1 cells was observed. Effect of polyphyllin Ⅶ on cell migration and invasion were detected by wound scratch assay and Transwell chamber. Effect of polyphyllin Ⅶ on apoptosis was detected by flow cytometry. Western blotting was used to detect the effect of polyphyllin Ⅶ on expressions of cysteine protease-3 (Caspase-3), B-cell lymphoma-2 (Bcl-2), Bcl-associated X protein (Bax), poly ADP-ribose polymerase (PARP), inhibitor of caspase-activated deoxyribonuclease (ICAD), and programmed death ligand 1 (PD-L1) in PANC-1 cells. Results Survival rate of PANC-1 cells was inhibited, migration and invasion of PANC-1 cells were significantly inhibited (P < 0.01), cell apoptosis was significantly induced (P < 0.05, 0.01), expressions of apoptosis-related proteins Cleaved Caspase-3, PARP, Bax were significantly increased (P < 0.01), and expressions of pro-PARP, ICAD, Bcl-2, and PD-L1 were significantly reduced by polyphyllin Ⅶ (P < 0.05, 0.01). Conclusion Polyphyllin Ⅶ can inhibit proliferation, migration and invasion of PANC-1 cells, and may induce PANC-1 cell apoptosis by down-regulating PD-L1 expression.
[中图分类号]
R285.5
[基金项目]
国家自然科学基金青年项目（81603265）；陕西省创新人才推进计划-青年科技新星项目（2019KJXX-057）；陕西省高校青年杰出人才支持计划（05041904）；陕西省科技厅面上项目（2021JM-489）；国家级大学生创新训练项目（201911840017）
