目的 以绿原酸和菊苣酸及浸出物含量为指标，建立菊苣Cichorium intybus叶最佳干燥方法；优选菊苣叶饮片的最佳炮制工艺，为菊苣叶的开发应用奠定基础。方法 建立菊苣叶中绿原酸和菊苣酸含量测定的HPLC方法，测定条件：色谱柱为Agilent Zorbax Eclipse XDB-C₁₈柱（250 mm×4.6 mm，5 μm），以乙腈-0.4%磷酸水溶液为流动相梯度洗脱，体积流量为1 mL/min，柱温为30℃，检测波长为334 nm；采用绿原酸和菊苣酸及浸出物多指标确定菊苣叶最佳干燥方法；采用星点设计-响应面法对菊苣叶切制段长、干燥温度、干燥时间进行考察，以绿原酸、菊苣酸、浸出物含量及色度值、饮片外观性状等按照权重计分为评价指标并进行综合分析。结果 菊苣叶最佳干燥方法为远红外干燥法；菊苣叶饮片炮制工艺条件为切制段长7 mm、干燥温度为50℃、干燥时间为17 h。结论 优选出的菊苣叶炮制工艺综合评分较高，不仅最大限度保留有效成分，而且饮片色泽好，可重复性强，工艺稳定可靠。

Objective To establish the best drying method for Cichorium intybus leaves based on the content of chlorogenic acid and cichoric acid and extracts; and optimize the best processing technology of C. intybus leaves, to lay a foundation for the development and application of C. intybus leaves. Methods An HPLC method for the simultaneous determination of chlorogenic acid and cichoric acid was established, the chromatographic column was an Agilent Zorbax Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm) with acetonitrile (A)-0.4% phosphoric acid water (B) as mobile phase gradient elution. The flow rate was 1 mL/min, the column temperature was 30℃, and the detection wavelength was 334 nm. The optimum drying method of C. intybus leaves was determined by chlorogenic acid, cichoric acid, and extracts multi-index comparison. The cutting length, drying temperature, and drying time of chicory leaves were investigated by central composite design-response surface methodology. The content of chlorogenic acid, cichoric acid, and the crude extract and the color value, and appearance properties of decoction pieces were comprehensively analyzed as evaluation indicators according to weight. Results The best drying method for chicory leaves was far-infrared drying. The processing conditions of C. intybus leaves pieces were as follows:the cutting length was 7 mm, the drying temperature was 50℃, and the drying time was 17 h. Conclusion The optimized processing technology of C. intybus leaves has a high comprehensive score, not only retains the active ingredients to the maximum extent but also has a good color, reproducibility, stable and reliable process.

R283.6

公益性行业专项-中药炮制技术传承基地建设（00104296）