目的 筛选并优化黄白温敏型原位凝胶的处方，并考察其经皮渗透性能。方法 以泊洛沙姆407（P407）、泊洛沙姆188（P188）、聚乙二醇6000（PEG6000）作为凝胶基质材料，以胶凝温度为考察指标，采用Box-Behnken效应面法优化温敏凝胶处方；对制备的温敏凝胶进行理化表征，并采用Franz透皮扩散池法考察黄白温敏凝胶的经皮渗透性能。结果 黄白温敏型原位凝胶的最佳处方为P407 20.10%，P188 2.54%，PEG6000 2.00%，胶凝温度为（34.2±0.2）℃，24 h内欧前胡素、盐酸小檗碱的累积渗透量分别为（14.07±1.34）、（395.72±35.04）μg/cm²，均符合Higuchi动力学方程。结论 制备的凝胶具有温敏、缓释作用，为临床提供治疗蛇虫咬伤的制剂奠定基础。

Objective To select and optimize the Phellodendron chinense and Angelica dahurica (Yellow-white) thermosensitive in-situ gel, and to investigate its transdermal permeability. Methods Using poloxamer 407, poloxamer 188, and PEG6000 as gel matrix materials, and taking gelation temperature as investigation index, Box-Behnken response surface method was used to optimize the gel matrix prescription. At the same time, the thermosensitive gel was characterized, and the transdermal permeability of drugs in the yellow-white thermosensitive in-situ gel was investigated by Franz transdermal diffusion cell. Results The best prescription of thermosensitive in-situ gel is P407 20.10%, P188 2.54%, PEG6000 2.00%, and the gelation temperature is (34.2 ±0.2)℃. Within 24 h, the cumulative permeation of imperatorin and berberine were (14.07 ±1.34) and (395.72 ±35.04) μg/cm², respectively, and the release process of imperatorin and berberine followed Higuchi kinetic equation. Conclusion The gel prepared in this study has temperature sensitivity and slow release, which can lay the foundation for clinical preparation for treating snake and insects bites.

R283.6

国家自然科学基金地区基金项目（82060722）；江西省自然科学基金面上项目（20202BAB206081）