目的 研究夹竹桃科山橙属植物山橙Melodinus suaveolens茎中的非生物碱类化学成分。方法 综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等色谱学方法进行系统分离和纯化，根据化合物的理化性质及其波谱学数据，并通过与文献中报道的波谱数据进行对照，鉴定化合物的化学结构。结果 从山橙茎的90%乙醇提取物的石油醚萃取部位中分离得到了18个非生物碱类化合物，分别鉴定为lyoniresinol（1）、4-epi-larreatricin（2）、curcasinlignan B（3）、pinnatifidanin C I（4）、threo-guaiacylglycerol- 8-O-4'-（coniferyl alcohol） ether（5）、cephafortin B（6）、vladinol D（7）、tripterygiol（8）、蔷薇酸（9）、30-醛基羽扇豆醇（10）、古柯二醇（11）、β-香树酯酮（12）、豆甾-7-烯-3β-醇（13）、豆甾-3，6-二酮（14）、α-波菜甾酮（15）、blumenol A（16）、4，5-dihydroblumenol（17）和去氢催吐萝芙木醇（18）。其中化合物1~8为木脂素类化合物，化合物9~12为三萜类化合物，化合物13~15为甾体类化合物，16~18为倍半萜类化合物。结论 所有化合物均为首次从山橙属植物中分离得到。

Objective To study the non-alkaloid constituents from the stems of Shancheng (Melodinus suaveolens). Methods The non-alkaloid constituents from the stems of M. suaveolens were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as the comparisons with the data reported in literature. Results A total of 18 compounds were isolated from the petroleum ether extract of the 90% ethanol extract of the stems of M. suaveolens, which were identified as lyoniresinol (1), 4-epi-larreatricin (2), curcasinlignan B (3), pinnatifidanin C I (4), threo-guaiacylglycerol-8-O-4'-(coniferyl alcohol) ether (5), cephafortin B (6), vladinol D (7), tripterygiol (8), euscaphic acid (9), 30-oxo-lupeol (10), erythrodiol (11), β-amyrenone (12), stigmast-7-en-3β-ol (13), stigmastan-3, 6-dione (14), α-spinasterone (15), blumenol A (16), 4, 5-dihydroblumenol (17) and dehydrovomifoliol (18). Among these isolated compounds, compounds 18 are lignans, compounds 9-12 are triterpenoids, compounds 13-15 are steroids, and 16-18 are sesquiterpenes. Conclusion All compounds are isolated from the genus Melodinus for the first time.

R284.1

国家自然科学基金资助项目（21662011）；国家级大学生创新创业训练计划项目（202011658003，202011658012）；海南省级大学生创新创业训练计划项目（S202011658009，S202011658029）；海口市重点研发计划项目（2020054）