以青蒿素为基础的联合疗法是疟疾的首选治疗方案，而药用植物黄花蒿Artemisia annua是青蒿素的唯一天然来源，也是目前最主要的青蒿素来源，因此培育高产青蒿素的黄花蒿一直是国际研究热点。青蒿素是黄花蒿的次生代谢产物，在植物的次生代谢物合成过程中，关键的转录因子可以调节代谢途径中某个或多个基因的表达，从而调节代谢流的方向和速度，决定着代谢物的产量，因此关键转录因子的表达对于青蒿素的合成非常重要，通过干预转录因子的表达也是提高青蒿素产量的重要手段。综述了黄花蒿中已研究的转录因子功能及调控机制，特别是对筛选获得转录因子基因的方法进行总结，以期为揭示青蒿素合成调控网络奠定基础。

Artemisinin-based combination therapy is the preferred treatment for malaria. The medicinal plant Artemisia annua is the only natural source and the main source of artemisinin. It is a hotspot to cultivate a strain of A. annua with high artemisinin content. Artemisinin is a secondary metabolite of A. annua. In the process of plant secondary metabolism, transcription factors play important roles in regulating a series of genes in the metabolic pathway, thereby regulating the direction and speed of metabolic flow. Therefore, the intervention of transcription factor by genetic engineering is an important method to regulate plant secondary metabolism. This article summarizes the functions and regulatory mechanism of transcription factors had been studied in A. annua. In particular, the methods for screening these transcription factors genes were summarized in order to provide a reference for the finding of key functional genes.

R282.12

国家自然科学基金资助项目（81673529）；科技部新药创制重大专项（2017ZX09101002-003-002）