目的 研究伊犁贝母Fritillaria pallidiflora的化学成分及其体外抗肿瘤活性。方法 应用多种色谱方法进行分离纯化，利用NMR、MS等波谱技术结合理化性质进行结构鉴定；并采用MTT法评价化合物对子宫颈癌HeLa细胞、结肠癌Ht-29细胞和人恶性黑色素瘤A375细胞的体外抑制活性。结果 从伊犁贝母95%乙醇提取物中分离得到13个化合物，分别鉴定为1-O-阿魏酰单甘油酯（1）、4-羟基苯甲醛（2）、环（D）-脯-（D）-苯丙（3）、香草酸（4）、2-羟基-3-苯基丙酸甲酯（5）、灯盏花苷II （6）、反式肉桂酸甲酯（7）、西贝母碱（8）、阿魏酸乙酯（9）、贝母辛（10）、环（脯氨酸-甘氨酸）（11）、西贝母碱苷（12）、6，7-二甲氧基-2-萘乙酸（13）。结论 化合物1~7、9、11、13为首次从伊犁贝母属中分离得到；化合物3、8、10~12对3种肿瘤细胞株具有不同程度的抑制活性，其中化合物10活性较强，其半数抑制浓度（IC₅₀）分别为22.07、52.47、48.27 μmol/L。

Objective To study the chemical constituents from Fritillaria pallidiflora and their antitumor activities. Methods Separation and purification of 95% ethanol extract of F. pallidiflora were carried out by using silica gel column, gel column and pre-HPLC and other chromatographic techniques, using NMR, MS, and other spectral techniques combined with physical and chemical properties were used for structural identification; In vitro tumor cell inhibitory activities of constituents to cervical cancer cells (HeLa), colon cancer cells (Ht-29) and human malignant melanoma cells (A375) were evaluated. Results Thirteen chemical constituents, including 1-O-feruloylglycerol (1), 4-hydroxybenzaldehyde (2), cyclo(D)-pro-(D)-phe (3), vanillic acid (4), 2-hydroxy-3-phenylpropanoic acid methyl ester (5), erigeside II (6), methyl cinnamate (7), imperialine (8), ethyl 3-(4-hydroxy-3-methoxyphenyl)acrylate (9), peimisine (10), cyclo(pro-gly) (11), sipeimine-3β-D-glucoside (12), and 6,7-dimethoxynaphthalene-2-carboxylic acid (13) were isolated from F. pallidiflora. Conclusion Compounds 1-7, 9, 11, and 13 are isolated from F. pallidiflora for the first time. And compounds 3, 8, 10-12 exhibit inhibitory activity against the tested cell lines. The IC₅₀ of compound 10 to the tested cell lines were 22.07, 52.47, and 48.27 μmoL/L, respectively.

R284.1

2018年中医药公共卫生服务补助专项“全国中药资源普查项目”（财社[2018]43号）；国家中医药管理局全国中药资源普查项目（GZY-KJS-2018-004）