目的 研究大果藤黄Garcinia pedunculata果实的化学成分，以期发现新的活性化合物。方法 运用硅胶、Sephadex LH-20、Chromatorex C₁₈、Diol silica gel、MCI gel CHP-20P以及反相半制备型高效液相等各种现代色谱分离技术进行系统的分离纯化，根据化合物的光谱数据和理化性质进行结构鉴定。结果 从大果藤黄中共分离得到11个香豆素类化合物，分别鉴定为（S）-5-羟基-7-甲氧基-8-（2-羟基-3-甲基-3-丁烯-1-基）-2H-1-苯并吡喃-2-酮（1）、（S）-5-羟基-7-甲氧基-8-（2-羟基-3-乙氧基-3-甲基丁基）-2H-1-苯并吡喃-2-酮（2）、异欧前胡素（3）、橙皮油内酯（4）、（-）-3'-乙基-橙皮内酯水合物（5）、（+）-2'-羟基-异蛇床子素（6）、（+）-2'-乙酰基-3'-羟基-蛇床子素（7）、（-）-yuehgesin B （8）、异橙皮内酯（9）、（-）-橙皮内酯水合物I （10）、（-）-橙皮内酯水合物（11）。结论 化合物1、2是新香豆素类化合物，命名为（S）-5，2'-二羟基-异蛇床子素和橙皮内酯水合物V，以上香豆素类成分均在大果藤黄中首次分离得到。

Objective For the purpose of finding new bioactive compounds from natural resources, the phytochemical investigation on the fruit of Garcinia pedunculata was carried out. Methods The chemical constituents from G. pedunculata were isolated through various modern chromatographic separation techniques including silica gel, Sephadex LH-20, Chromatorex C₁₈, Diol silica gel, MCI gel CHP-20P, and semi-preparative HPLC. The structures of compounds were identified based on spectroscopic data and physicochemical properties. Results Eleven coumarins were isolated and identified as (S)-5-hydroxy-7-methoxy-8-(2-hydroxy-3- methyl-3-buten-1-yl)-2H-1-benzopyran-2-one (1), (S)-5-hydroxy-7-methoxy-8-(2-hydroxy-3-ethoxy-3-methylbutyl)-2H-1-benzopyran- 2-one (2), isoimperatorin (3), auraptene (4), (−)-3'-ethyl-meranzin hydrate (5), (+)-2'-hydroxyl-isoosthole (6) (+)-2'-acetoxy-3'- hydroxy-osthole (7), (−)-yuehgesin B (8), isomeramazin (9), (−)-meranzin hydrate I (10), and (−)-meranzin hydrate (11). Conclusion Compounds 1 and 2 are identified as two new coumarins named as (S)-5,2'-dihydroxy-isoothole and meranzin hydrate V.

R284.1

国家自然科学基金资助项目（8147311）