目的 探讨蚕蛾公补合剂调控Wnt/β-catenin信号通路改善精子活力和数量的机制。方法 研究分析天津市中医药研究院附属医院、天津中医药大学第一附属医院和中国中医科学院西苑医院门诊就诊的少弱精子症患者60例，其中治疗组30例（服用蚕蛾公补合剂治疗），对照组30例（服用五子衍宗丸治疗），2组治疗周期均为12周，检测患者精液常规指标，RT-PCR检测精液Wnt/β-链蛋白（β-catenin）mRNA表达以及Western blotting检测Wnt/β-catenin蛋白表达。结果 治疗组较对照组患者精液质量明显提高，Wnt/β-catenin mRNA及蛋白表达也明显增加。结论 蚕蛾公补合剂能够改善精子质量与活力，作用机制与Wnt/β-catenin信号通路相关。

Objective To explore the mechanism of Wnt/β-catenin signaling pathway regulating by Can'egongbu Mixture (蚕蛾公补合剂) to improve sperm motility and quantity. Method The study analyzed 60 patients with oligozoospermia attending the outpatient clinics of the Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine and Xiyuan Hospital CACMS, including 30 cases in the treatment group (treated with Can'egongbu Mixture) and 30 cases in the control group (treated with Wuzi Yanzong Pills, 五子衍宗丸). The treatment cycle of each group was 12 weeks, and the patients were tested for routine semen indicators, expression of Wnt/β-catenin mRNA in semen was detected by RT-PCR, and Western blotting was used to detect Wnt/β-catenin protein expression. Result Compared with the control group, the quality of semen in the treatment group was significantly improved, and the expressions of Wnt/β-catenin mRNA and protein were also significantly increased. Conclusion Can'egongbu Mixture can improve sperm quality and sperm motility, its mechanism of action is related to the Wnt/β-catenin signaling pathway.

R285

天津市卫生和计划生育委员会中西医结合课题（2017129）