目的 在石榴皮炭Granati Pericarpium Carbonisatum中发现并分离出新型纳米类成分，并通过动物实验来研究其止泻作用。方法 利用高温热解法对生石榴皮进行炭化，并对炭化产物进行萃取、滤过分离和透析得到一种具有水溶性的新型纳米类成分，命名为石榴皮炭纳米类成分（GPC-NCs）。采用透射电子显微镜、高分辨透射电子显微镜、傅里叶变换红外光谱、紫外光谱、荧光光谱、高效液相色谱、X射线光电子能谱和X射线衍射对GPC-NCs进行表征。使用小鼠腹泻模型观察小鼠腹泻指数、腹泻潜伏期和肠道传输功能，以此来评价和证实GPC-NCs的止泻作用。结果 从石榴皮炭中分离出来的GPC-NCs外形为类球形，粒径均匀分布在1.2～2.3 nm，晶格间距为0.321 nm，荧光量子产率为0.45%。GPC-NCs能够减少小鼠腹泻指数和腹泻潜伏期时间，并对番泻叶水煎剂所致的小肠运动功能亢进具有拮抗作用。结论 GPC-NCs的显著止泻效果，不仅为石榴皮炭的物质基础研究提供了一个新思路，也为GPC-NCs在止泻领域的潜在生物医学和医疗保健应用提供了新的见解。

Objective To discover and isolate a novel type of nano-components in Granati Pericarpium Carbonisatum (GPC), and evaluate its antidiarrheal effect through animal experiments. Methods The Shiliupi (Granati Pericarpium) was carbonized by high-temperature pyrolysis, and the carbonized products were extracted, filtered and separated to obtain a novel type of water-soluble nano-components, named GPC-NCs. GPC-NCs were characterized by transmission electron microscopy, high resolution transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet spectroscopy, fluorescence spectroscopy, high performance liquid chromatography, X-ray photoelectron spectroscopy and X-ray diffraction. The mouse diarrhea models were used to observe the mouse diarrhea index, diarrhea incubation period and intestinal transit function to evaluate and confirm the antidiarrheal effect of GPC-NCs. Results The GPC-NCs separated from the GPC were quasi-spherical in shape, with a uniform particle size distribution of 1.2-2.3 nm, a lattice spacing of 0.321 nm, and a fluorescence quantum yield of 0.45%. GPC-NCs could reduce the diarrhea index and the incubation period of diarrhea in mice, and had an antagonistic effect on small intestinal hypermotility caused by senna decoction. Conclusion These results indicate the significant antidiarrheal effect of GPC-NCs not only provides a new idea for the material basis study of GPC, but also provides new insights for the potential biomedical and healthcare applications of GPC-NCs in the field of antidiarrheal.

R283.6

中央高校基本科研业务费专项资金资助项目团队项目：经典方剂的应用基础研究（2019-JYB-TD-001）