目的 研究甘草汁蒸制前后高乌头Aconitum sinomontanum石油醚部位HPLC指纹图谱与行气药效之间的相关性，为明确甘草汁蒸制前后高乌头石油醚部位理气药效物质基础提供实验依据。方法 通过HPLC法建立10批不同产地生、制高乌头石油醚部位（S1～S10、Z1～Z10）指纹图谱；采用炭末推进法评价甘草汁蒸制前后高乌头石油醚部位的行气作用；同时采用灰色关联度分析方法，对7批生、制高乌头石油醚部位进行谱效相关性分析，找到各自药效贡献较大成分，评价二者相似性。结果 不同产地生、制高乌头石油醚部位有促进小鼠小肠运动的作用，其中以Z2、S3作用能力最强。甘草汁蒸制前后高乌头石油醚部位发挥行气作用的药效是多种成分共同作用的结果，其中生品高乌头石油醚部位各共有峰所代表的化学成分对其行气作用贡献的大小顺序为（关联度 > 0.8）40号峰 > 7号峰 > 30号峰 > 14号峰 > 36号峰 > 24号峰 > 13号峰 > 15号峰 > 33号峰 > 23号峰 > 41号峰 > 43号峰 > 9号峰 > 21号峰 > 28号峰 > 31号峰 > 37号峰 > 17号峰 > 34号峰；制品高乌头石油醚部位各共有峰所代表的化学成分对其行气作用贡献的大小顺序为（关联度 > 0.8）41号峰 > 29号峰 > 4号峰 > 27号峰 > 30号峰 > 33号峰 > 34号峰 > 20号峰 > 1号峰 > 45号峰 > 22号峰 > 19号峰 > 38号峰 > 31号峰 > 18号峰 > 14号峰 > 28号峰。结论 通过谱效关系研究获得了甘草汁蒸制前后高乌头石油醚部位的行气药效物质群，其行气药效是多种成分共同作用的结果，为探索甘草汁蒸制前后高乌头石油醚部位行气作用物质基础的研究提供了参考。

Objective To study the correlation between HPLC fingerprint of petroleum ether fraction and promoting qi effect of Wutou (Aconitum sinomontanum) before and after steaming with licorice juice, and provide experimental basis for clarifying the promoting qi material basis of petroleum ether fraction of A. sinomontanum before and after steaming of licorice juice. Methods HPLC method was applied to establish the fingerprint chromatography of 10 batches of raw and processed A. sinomontanum petroleum ether fraction at different habitats, and the effect of promoting qi of petroleum ether fraction of A. sinomontanum before and after steaming with licorice juice was evaluated by carbon powder propulsion method. At the same time, the method of grey correlation analysis was used to analyze the spectral correlation of seven batches of raw and processed A. sinomontanum petroleum ether fractions, so as to find the ingredients with greater contribution to efficacy and evaluate the similarities. Results The raw and processed A. sinomontanum petroleum ether fraction at different habitats could promote the movement of small intestine in mice, among which Z2 and S3 had the strongest effect. The effect of promoting qi of petroleum ether fraction of A. sinomontanum before and after steaming with licorice juice was the result of the joint action of many components. Among them, the order of the contribution of the chemical components of petroleum ether fraction of raw A. sinomontanum for promoting qi at the common peaks was as follow (r > 0.8):P40 > P7 > P30 > P14 > P36 > P24 > P13 > P15 > P33 > P23 > P41 > P43 > P9 > P21 > P28 > P31 > P37 > P17 > P34. The order of the contribution of the chemical components of petroleum ether fraction of processed A. sinomontanum for promoting qi at the common peaks was as follow (r > 0.8):P41 > P29 > P4 > P27 > P30 > P33 > P34 > P20 > P1 > P45 > P22 > P19 > P38 > P31 > P18 > P14 > P28. Conclusion Through the study of spectrum-effect relationship, the active substance group of A. sinomontanum petroleum ether fraction before and after steaming with licorice juice was obtained, and its promoting qi effect was the result of the joint action of many components. It provides a reference for exploring the material basis of petroleum ether fraction of A. sinomontanum for promoting qi before and after steaming with licorice juice.

R283.6

国家自然科学基金项目（81560650）；全国中药特色技术传承人才培训项目（T20184828005）