目的 在以有效相态为视角的中药药性传递理论指导下完成生脉饮有效相态拆分、表征及药效学验证，指认生脉饮对气阴两虚型SD大鼠心力衰竭模型治疗的有效迭代相态。方法 采用膜透析及离心等方法，进行生脉饮有效相态拆分；通过马尔文激光粒度仪对拆分后的生脉饮各相态及生脉饮全溶液进行粒径、Zeta电位、PDI值测定，以及采用盐度仪及电导率仪对拆分后的生脉饮全溶液各相态及生脉饮全溶液的盐度和电导率进行测定，完成生脉饮有效相态表征研究；在气阴两虚型SD大鼠心力衰竭模型上验证生脉饮的药效学研究。结果 各相态的粒径大小的分布规律为混悬相态>生脉饮全溶液>胶体相态>真溶液；PDI的大小排列顺序为生脉饮全溶液>混悬相态>胶体相态>真溶液；各相态Zeta电位值的排列顺序为真溶液>胶体相态>生脉饮全溶液>混悬相态；各相态电导率的大小排列顺序为生脉饮全溶液>混悬相态>胶体相态>真溶液；各相态盐度的大小排列顺序为混悬相态>生脉饮全溶液>胶体相态>真溶液。生脉饮全溶液组和胶体相态组大鼠的各项指标与模型组大鼠比较均有明显改善（P<0.05），而真溶液组和混悬相态组大鼠的各项指标与模型组比较没有显著性差异，证实胶体相态为生脉饮全溶液的有效迭代相态。结论 在中药药性传递理论指导下，以药效学研究为评价指标，以有效相态为视角进行生脉饮有效迭代相态指认，进而以有效迭代相态为评价指标指导新药开发，可为中药新药开发提供一种新的思路。

Objective To complete the effective phase state separation, characterization and pharmacodynamic verification of Shengmai Yin (Pulse-activating Decoction, 生脉饮) under the guidance of the traditional Chinese medicines (TCM) pharmaceutical delivery theory from the perspective of effective phase, and to identify the effective iterative phase of treatment of Shengmai Yin on the heart failure model of SD rats with deficiency of both qi and yin. Methods Membrane dialysis and centrifugation were used to carry out effective phase separation of Shengmai Yin; The separated phases, particle size, Zeta potential, and PDI of solution were measured by using Malvern laser particle size analyzer. The salinity and conductivity of the separated Shengmai Yin solution were measured by the salinity meter and the conductivity meter to complete the study on the effective phase behavior of Shengmai Yin; The pharmacodynamic study of Shengmai Yin was verified on the SD rat heart failure model with deficiency of both qi and yin. Results The particle size distribution of each phase was:suspension phase > Shengmai Yin solution > colloidal phase > true solution; The order of PDI was:Shengmai Yin solution > suspension phase > colloidal phase > true solution; The order of Zeta potential values of each phase was:true solution > colloid phase > Shengmai Yin solution > suspension phase; The order of conductivity of each phase was:Shengmai Yin solution > suspension state > colloidal state > true solution; The order of the salinity of each state was:suspension state > Shengmai Yin solution > colloidal state > true solution. The indexes of the rats in the Shengmai Yin solution and the colloidal phase group were significantly improved compared with those of the model group (P<0.05), but the indexes of the true solution group and the suspension phase group were not significant compared with the model group. The difference proved that the colloidal phase was the effective iterative phase state of Shengmai Yin solution. Conclusion Under the guidance of the pharmaceutical delivery theory of TCM, the pharmacodynamic research is used as the evaluation index, and the effective iterative phase of Shengmai Yin is identified from the perspective of the effective phase, and then the effective iterative phase is used as the evaluation index to guide the development of new medicine, which can provide a new way of thinking for development of new medicine in traditional Chinese medicine.

R283.6

国家自然科学基金面上项目（81703719）；黑龙江省博士后特别资助（博士后青年英才计划，LBH-TZ1620）；黑龙江省博士后科研启动基金项目（LBH-Z11276）；黑龙江省博士后科研启动基金项目（LBH-Q16215）；黑龙江省自然科学基金项目（H2018050）；黑龙江省自然科学基金项目（LH2019H102）；黑龙江中医药大学校基金项目（201831）；黑龙江中医药大学校基金项目（2019MS40）