目的 探究苏木Caesalpinia sappan的活性成分苏木酮A对大鼠类风湿性关节炎（rheumatoid arthritis，RA）的治疗作用。方法 以牛Ⅱ型胶原蛋白诱导建立RA大鼠模型，以苏木酮A（50 mg/kg）进行干预，考察大鼠足容积变化，对大鼠踝关节组织进行病理分析，采用免疫组化考察大鼠踝关节组织中白细胞介素-6（interleukin-6，IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）和基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）表达。以脂多糖诱导Raw264.7细胞建立炎症细胞模型，考察苏木酮A对Raw264.7细胞上清液中一氧化氮和IL-6水平的影响。结果 苏木酮A显著降低RA大鼠足容积（P<0.05），改善RA大鼠踝关节滑膜组织损伤，抑制RA大鼠踝关节滑膜组织中IL-6、TNF-α和MMP-9的表达。苏木酮A显著抑制脂多糖诱导的Raw264.7细胞上清液中一氧化氮和IL-6水平（P<0.01）。结论 苏木酮A可有效缓解大鼠RA。

Objective To investigate the effect of sappanone A, an effective active ingredient from Caesalpinia sappan on rheumatoid arthritis in rats. Methods Bovine type II collagen was used to induce rheumatoid arthritis rats model and sappanone A (50 mg/kg) was used to intervention. Changes of rat foot volume were detected and pathological analysis of rat ankle joint tissue was carried out. Expressions of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and matrix metalloproteinase-9 (MMP-9) in rat ankle joint tissue were investigated by immunohistochemistry. Lipopolysaccharide was used to induce Raw264.7 cells to establish an inflammation cell model. Levels of nitric oxide and IL-6 in the supernatant of Raw264.7 cells were detected. Results Sappanone A significantly reduced the foot volume of RA rats (P<0.05), improved the damage of the ankle synovial tissue of RA rats, and inhibited the expressions of IL-6, TNF-α, and MMP-9 in the ankle synovial tissue of RA rats. Sappanone A significantly inhibited the levels of nitric oxide and IL-6 in the supernatant of Raw264.7 cells induced by lipopolysaccharide (P<0.01). Conclusion Sappanone A could effectively alleviate RA in rats.

R285.5

国家自然科学基金资助项目（81973505）；国家重点研发计划基金资助项目（2019YFC1708902，2019YFC1711000）