目的 建立补骨脂Psoralea corylifolia药材的UPLC指纹图谱，同时对其中12种成分进行含量测定，为其质量控制提供参考。方法 采用Shim-pack GIST-HP色谱柱（100 mm×2.1 mm，1.9 μm）；以水和乙腈为流动相，梯度洗脱，建立10个产地29个批次的补骨脂药材的UPLC指纹图谱，并采用中药色谱指纹图谱相似度评价软件（2012年版）进行相似度评价。同时测定了补骨脂药材中12种成分的含量。结果 建立了补骨脂药材的UPLC指纹图谱，29批药材的相似度在0.827~0.989，共标定出21个共有峰，并指认出其中12个色谱峰。定量分析条件方法学考察结果良好，29批药材中补骨脂素、异补骨脂素、异补骨脂二氢黄酮、新补骨脂异黄酮、补骨脂甲素、欧前胡素、补骨脂宁、补骨脂定、4，5-去氢异补骨脂定、补骨脂乙素、异补骨脂色烯查耳酮、补骨脂酚的质量分数依次为1.08~6.95、0.76~5.01、0~0.62、0.40~2.54、0.32~1.75、0~0.25，0.06~0.73、0.23~1.83、0~0.27、0.86~6.86、0.08~1.59、4.20~20.76 mg/g。结论 UPLC指纹图谱结合多成分同时测定的方法，操作简便，结果准确、稳定，可为补骨脂药材的质量评价提供参考。

Objective To establish the UHPLC fingerprint and simultaneously determinate 12 components of the standard decoction of Psoraleae Fructus, so as to provide reference for the quality control of Psoraleae Fructus. Methods The UHPLC fingerprints of Psoraleae Fructus from 10 regions and 29 batches was performed on Shim-pack GIST-HP chromatographic column (100 mm×2.1 mm, 1.9 μm) with the mobile phase consisting of acetonitrile and water. Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012 edition) was used to evaluate the fingerprints. At the same time, the contents of 12 active components were determined. Results The UHPLC fingerprint of 29 batches of Psoraleae Fructus was established. The similarity was between 0.827-0.989, and 21 common peaks were marked. Twelve peaks were confirmed. The results of quantitative analysis were good. The content of psoralen, isopsoralen, bavachin, prenyldaidzein, coryfolin, imperatorin, corylin, psoralidin, 4,5-dehydroisopsoralidin, corylifolinin, isobavachromene, and bakuchiol in 29 batches of samples were in the range of 1.08-6.95, 0.75-5.01, 0-0.62, 0.40-2.54, 0.32-1.75, 0-0.25, 0.06-0.73, 0.23-1.83, 0-0.27, 0.86-6.86, 0.08-1.59 and 4.20-20.76 mg/g, respectively. Conclusion The combination methods of UHPLC fingerprint and simultaneous determinations of multiple components are rapid, simple and reproducible, which can provide reference for the quality evaluation of Psoraleae Fructus and its preparation.

R286.2

国家自然科学基金资助项目（81673826）；国家自然科学基金资助项目（81703790）