目的 研究3种新型红芪多糖（HPS-1、HPS-2、HPS-3）对大鼠骨髓间充质干细胞rBMSCs和颅骨成骨细胞ROBs成骨性分化的影响。方法 采用MTT法检测rBMSCs细胞和ROBs细胞增殖；采用碱性磷酸酶（alkaline phosphatase，ALP）试剂盒检测rBMSCs细胞和ROBs细胞的ALP活性；采用茜素红染法检测rBMSCs和ROBs细胞矿化结节的形成情况。结果 HPS-1（20、50 μg/mL）作用48 h显著促进rBMSCs细胞增殖（P<0.05、0.01），HPS-1（20 μg/mL）作用48 h显著促进ROBs细胞增殖（P<0.01）；HPS-2和HPS-3对2种细胞的增殖无促进作用。HPS-1（50、100 μg/mL）显著升高rBMSCs细胞的ALP活性（P<0.01），HPS-1（10、20、50 μg/mL）显著升高ROBs细胞的ALP活性（P<0.05、0.01）；HPS-3（50 μg/mL）显著升高rBMSCs细胞的ALP活性（P<0.05），HPS-2对2种细胞ALP活性没有促进作用。HPS-1显著增加rBMSCs和ROBs细胞中钙化结节面积和数量（P<0.01），HPS-2和HPS-3对rBMSCs和ROBs细胞钙化结节的形成没有促进作用。结论 HPS-1可增强rBMSCs和ROBs细胞ALP活性和成骨相关因子表达，并显著促进rBMSCs和ROBs细胞分化、矿化。

Objective To investigate the effects of hedysarum polysaccharides (HPS-1, HPS-2, HPS-3) on promoting osteogenic differentiation of rat bone marrow stromal cells (rBMSCs) and rat calvarial osteoblasts (ROBs). Methods MTT assay was used to detect the proliferation of rBMSCs and ROBs. Alkaline phosphatase (ALP) activity of rBMSCs and ROBs were detected by ALP kit. Mineralized nodule formation of rBMSCs and ROBs was detected by alizarin red S staining. Results HPS-1 (20, 50 μg/mL) significantly promoted the proliferation of rBMSCs (P < 0.05, 0.01), HPS-1 (20 μg/mL) significantly promoted the proliferation of ROBs (P < 0.01), HPS-2 and HPS-3 had no influence on promoting the proliferation of rBMSCs and ROBs. HPS-1 (50, 100 μg/mL) significantly increased ALP activity in rBMSCs (P < 0.01), HPS-1 (10, 20, 50 μg/mL) significantly increased ALP activity in ROBs (P < 0.05, 0.01), HPS-3 (50 μg/mL) significantly increased ALP activity in rBMSCs (P < 0.05). HPS-2 had no influence on increasing ALP activity in rBMSCs and ROBs. HPS-1 significantly increased the area and number of calcified nodules of rBMSCs and ROBs (P < 0.01), HPS-2 and HPS-3 had no influence on promoting the formation of calcified nodules of rBMSCs and ROBs.Conclusion HPS-1 could enhance the cell activity and osteogenesis-related factor expression, and significantly promote the differentiation and mineralization of rBMSCs and ROBs cells.

R285.5

国家自然科学基金资助项目（81703664）