目的 研究青蒿（黄花蒿Artemisia annua）干燥地上部分的化学成分。方法 综合应用HP-20、硅胶、ODS、Sephadex LH-20和半制备液相等多种色谱学方法进行分离纯化，并根据化合物的理化性质和核磁共振波谱数据进行结构鉴定。结果 从青蒿干燥地上部分水提取物中分离得到了5个化合物，分别鉴定为 （S）-2，6，11-trimethyl-7，8-dihydro-6H-cyclohepta[b] pyridine-9-carboxylic acid（1）、arteannoide E（2）、蚱蜢酮（3）、（6S，9S）-长寿花糖苷（4）、artemanin B（5）。结论 化合物1为新的生物碱，命名为青蒿生物碱；化合物3为首次从蒿属植物中分离得到。

Objective To study the constituents from the dried aboveground part of Artemisia annua. Methods The compounds were isolated and purified by column chromatography of HP-20 macroporous resin, silica gel, ODS, Sephadex LH-20, and semi-preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Results Five compounds were isolated and identified from the water extract of A. annua, including alkaloid; (S)-2,6,11-trimethyl-7,8- dihydro-6H-cyclohepta[b]pyridine-9-carboxylic acid (1), arteannoide E (2), grasshopper ketone (3), (6S,9S)-roseoside (4) andartemanin B (5). Conclusion Compound 1 is a new alkaloid named arteannine. Compound 3 is identified from Artemisia genus for the first time.

重大新药创制科技重大专项“十三五”计划（2018ZX09711001-008-005）；国家自然科学基金重点项目（81630097）