目的 研究粗茎秦艽Gentiana crasicaulis种子萌发的转录组信息及影响因子。方法 利用高通量测序技术测定粗茎秦艽种子萌发前、萌发中及萌发后3个阶段的转录组，并通过生物信息学技术进行分析。结果 共获得了149 463条Unigenes，平均长度为601.88 bp，其中79 412个Unigene获得注释。6943个基因差异表达，大部分表现为萌发后上调表达，其中5188个富集到GO条目中，1815个富集到KEGG通路中，大多参与光反应过程、细胞壁合成、脂质代谢及次生代谢中。单碱基到六碱基核苷酸的SSR重复类型均有检出，SSR发生频率为15.13%，出现频率为18.81%，平均每3199 bp就含有1个SSR位点，SSR重复类型丰富，数目较多。结论 粗茎秦艽种子萌发过程有大量调控基因参与，光照、激素为重要的调控因子，SSR专用标记的开发切实可靠，且为粗茎秦艽的次生代谢调控研究奠定了基础。

Objective To explore the transcriptome information and key factors of G. crasicaulis in seed germination process. Methods The transcriptome of the stage before germination, during germination and after germination of G. crasicaulis were determined by high-throughput sequencing and analyzed by systemic bioinformatics. Results A total of 149 463 unigenes were obtained, with an average length of 601.88 bp, and 79 412 unigenes were successfully annotated. A total of 6943 genes were differentially expressed, most of which were up-regulated after germination. Among them, 5188 genes were enriched in the GO and 1815 genes were enriched in the KEGG pathway, most of which were involved in the light reaction, cell wall synthesis, lipid metabolism and secondary metabolism. The 1-6 nucleotides SSR repeat types were all detected. The frequency of SSR occurrence was 15.13% and the frequency of appearing was 18.81%. On average, every 3199 bp contained one SSR site. Abundant SSR repeat types and a large number of SSR repeat types were obtained. Conclusion A large number of genes were involved in regulating the germination process of G. crasicaulis. Light and plant hormones were important regulatory factors. The special SSR markers development was effective and reliable through transcriptome information. This study provided a reference for further understanding of regulation of G. crasicaulis secondary metabolism.

R282.12

国家重点研发计划（2017YFC1700705）；四川省留学人员科技活动项目择优资助经费计划（2018-68）；四川省重点研发项目（2019YFS0174）；四川省科技支撑项目（14ZC2103）；西南民族大学研究生创新型科研项目（CX2020SZ77）