目的 研究异黄芪甲苷（6-O-β-D-葡萄糖基-环黄芪醇）对体外淋巴细胞和树突状细胞功能的影响。方法 培养巨噬细胞，观察异黄芪甲苷对细胞吞噬中性红能力和白细胞介素-1（interleukin-1，IL-1）、IL-2、IL-6分泌功能的影响。MTT法检测异黄芪甲苷对原代培养脾淋巴细胞增殖的影响，并用流式细胞术通过检测CD3、CD19水平，明确异黄芪甲苷分别对T、B淋巴细胞增殖的影响。进一步观察异黄芪甲苷对树突细胞增殖和分泌IL-6、IL-12 p70能力的影响。结果 异黄芪甲苷低、中、高3个剂量组可升高巨噬细胞的吞噬功能和分泌IL-6的能力，降低CD3⁺的比例，升高CD19⁺的比例，纠正T、B淋巴细胞的紊乱。中、高剂量组可显著升高IL-2水平，增加淋巴细胞的增殖，提高增殖指数（SI），促进树突状细胞（dendritic cells，DC）分泌IL-6和IL-12 p70。高剂量组还可升高巨噬细胞分泌IL-1的能力。结论 异黄芪甲苷的免疫促进作用机制与促进非特异性免疫吞噬，促进抗原递呈、淋巴细胞增殖和树突细胞分泌能力增强有关。

Objective To study the effect of iso-astragaloside VI (6-O-β-D-glucosyl-cycloastragenol) on the function of lymphocytes in vitro and dendritic cells. Methods The macrophages were cultured, and the effects of iso-astragaloside VI on helping to devour neutral red and secrete IL-1, IL-2 and IL-6 were also observed. Its effects on proliferating of primary cultured spleen lymphocytes were measured, along with the measurements of CD3 and CD19 by flow cytometry to determine its effects on T and B lymphocytes proliferations. In addition, its effects on promoting the proliferation and secretion of IL-6 and IL-12 p70 in dendritic cells (DC) were also observed. Results The three groups (low, medium and high doses) of iso-astragaloside VI increased the abilities of macrophages on phagocytosis and secretion of IL-6, improved T and B lymphocytes ratios by decreasing CD3⁺ and enhancing CD19⁺. Medium and high doses of iso-astragaloside VI elevated IL-2 level, lymphocyte proliferation and SI and promoted secretion of IL-6 and IL-12 p70 from DC. Additionally, the high dose group had the ability to increase the production of IL-1. Conclusion The potential mechanisms of iso-astragaloside VI on immunoenhancement can promote nonspecific immune phagocytosis, antigen presentation, lymphocyte proliferation and DC secretion.

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国家重点研发计划项目（2019YFC1605800）；国家自然科学基金项目（81973726）；江苏省自然科学基金项目（BK20150088）