目的 研究左金丸醇提物对人胃癌细胞SGC-7901增殖和糖酵解的作用及机制。方法 采用CCK-8法检测左金丸醇提物（5、10、25、50、100、200 μg/mL）对SGC-7901细胞增殖的影响；检测左金丸醇提物对SGC-7901细胞葡萄糖摄取量、乳酸和三磷酸腺苷（adenosine triphosphate，ATP）含量的影响，评价左金丸醇提物对SGC-7901细胞糖酵解的作用；采用qRT-PCR和Western blotting法检测左金丸醇提物对细胞糖酵解途径相关蛋白Rhotekin（RTKN）表达的影响；采用RTKN shRNA干扰慢病毒感染SGC-7901细胞，检测左金丸醇提物对SGC-7901细胞葡萄糖摄取、乳酸和ATP含量的影响。结果 左金丸醇提物对SGC-7901细胞增殖具有显著抑制作用（P<0.001），呈剂量、时间相关性；左金丸醇提物显著降低SGC-7901细胞葡萄糖摄取、乳酸和ATP含量（P<0.01、0.001）；左金丸醇提物显著降低SGC-7901细胞中RTKN mRNA和RTKN蛋白表达（P<0.001）；干扰RTKN抵消了左金丸醇提物对SGC-7901细胞葡萄糖摄取、乳酸和ATP含量的抑制作用（P<0.001）。结论 左金丸醇提物可显著抑制SGC-7901细胞增殖、降低细胞糖酵解水平，其机制与下调RTKN表达相关。

Objective To investigate the effect of Zuojin Pills (左金丸) alcohol extract on proliferation and glycolysis of gastric cancer SGC-7901 cells by downregulating Rhotekin (RTKN). Methods CCK-8 was used to evaluate the effect of Zuojin Pills alcohol extract (10, 25, 50, 100, 200 μg/mL) on cell viability of SGC-7901 cell. Glucose uptake, lactate and ATP content of SGC-7901 cells were detected to evaluate the effects of Zuojin Pills alcohol extract on glycolysis of SGC-7901 cells. qRT-PCR and Western blotting were used to detect glycolysis related protein RTKN expression. Moreover, RTKN shRNA were transfected into SGC-7901 cells to knockdown RTKN and the effects of Zuojin Pills alcohol extract on glucose uptake, lactate and ATP content were evaluated. Results Zuojin Pills alcohol extract exhibited a dose- and time-dependent anti-proliferative effect on SGC-7901 cells (P<0.001). In addition, glucose uptake, lactate and ATP content of SGC-7901 cells were significantly decreased (P<0.01, 0.001) with Zuojin Pills alcohol extract treatment. Zuojin Pills alcohol extract significantly reduced RTKN mRNA and RTKN protein expression in SGC-7901 cells (P<0.001). However, RTKN knockdown significantly attenuated the inhibitory effect of Zuojin Pills alcohol extract on glycolysis of SGC-7901 cells (P<0.001). Conclusion Zuojin Pills alcohol extract significantly inhibited the proliferation and glycolysis of SGC-7901 cells, which was related to the downregulation of RTKN.

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国家自然科学基金资助项目（81973808）；上海市自然科学基金资助项目（19ZR1447800）；上海中医药大学预算内项目（2019LK038）