[关键词]
[摘要]
目的 从肠道菌群角度，评价两亲性嵌段共聚物用于中药汤剂抑苦的适宜性和安全性。方法 以苦味强烈的盐酸小檗碱（berberine hydrochloride，BH）溶液为模型药物，采用高通量测序技术，对比研究两亲性嵌段共聚物、天然甜味剂（蔗糖）与人工甜味剂（阿斯巴甜）连续给药2周后对大鼠肠道菌群的影响。结果 BH经过两亲性嵌段共聚物、蔗糖、阿斯巴甜掩味后，其物种总数有升高的趋势。两亲性嵌段共聚物不影响BH菌群的多样性与群落构成；蔗糖具有使BH菌群多样性升高的趋势，阿斯巴甜具有使BH菌群多样性降低的趋势。结论 在长期服药条件下，两亲性嵌段共聚物作为汤剂抑苦辅料不干扰药物对肠道菌群的作用，对于儿童用药是一种更为安全的掩味剂。
[Key word]
[Abstract]
Objective To evaluate the suitability and safety of amphiphilic block copolymers for bitter suppression of traditional Chinese medicine decoction based on intestinal flora. Method Berberine hydrochloride (BH) with strong bitterness was used as model drug, the effects of amphiphilic block copolymer, natural sweetener (sucrose) and artificial sweetener (aspartame) on intestinal microflora of rats were compared after 2 weeks of continuous administration by high throughput sequencing technology. Result After being masked by amphiphilic block copolymers, sucrose and aspartame, the total number of species of BH was increased. The results showed that amphiphilic block copolymers did not affect the diversity and community composition of BH. Sucrose had a tendency to increase the diversity of BH flora, while aspartame had a tendency to decrease it. Conclusion Amphiphilic block copolymers do not interfere with the effect of the drug on intestinal flora in long-term medication, so it is a safe taste masking agent for children.
[中图分类号]
R283.6
[基金项目]
国家自然科学基金项目（81803745）；四川省科技厅青年杰出科技人才资助项目（2019JDJQ0007）；2019-2021年度中华中医药学会青年人才托举工程项目（2019-QNRC2-B05）；江西中医药大学现代中药制剂教育部重点实验室开放基金（TCM-201904）
