目的 研究外源海藻糖对NaCl胁迫下甘草Glycyrrhiza uralensis幼苗的生长及总黄酮含量的影响。方法 研究了NaCl胁迫对甘草幼苗生理生长、酶活性、离子含量、渗透调节及总黄酮含量的影响，采用Microsoft Excel 2010对数据进行处理分析与整理，以SPSS19.0统计软件对数据进行方差分析。结果 10～20 mmol/L海藻糖能够显著降低NaCl对甘草幼苗的伤害，且外源海藻糖浓度为15 mmol/L时的作用效果最好。NaCl胁迫下施加外源海藻糖浓度为15 mmol/L时甘草幼苗的生长最旺盛其生长量增加最明显，且影响甘草幼苗渗透调节的K⁺、K⁺/Na⁺浓度增加最明显，Na⁺和Cl^-的浓度相对于无NaCl胁迫时有所降低；外源海藻糖浓度为15 mmol/L时可使NaCl胁迫下甘草幼苗内的抗氧化酶活性提高，并且可以增加NaCl胁迫下植物细胞内光和色素叶绿素的含量，外源海藻糖浓度为15 mmol/L可以降低NaCl胁迫下甘草幼苗内可溶性糖、脯氨酸以及细胞调节物质丙二醛（MDA）的含量。结论 甘草在NaCl胁迫下施加适宜浓度的外源海藻糖能够促进甘草幼苗生长和有效成分的积累，减少了盐害对甘草生长的危害，增强NaCl胁迫下甘草的生长能力。

Objective To study the effects of exogenous trehalose on the growth and total flavonoids content of licorice (Glycyrrhiza uralensis) seedlings under NaCl stress. Methods Using licorice seedlings as the material, the effects of NaCl stress on physiological growth, enzyme activity, ion content, osmotic regulation and total flavonoid content of licorice seedlings were studied in this experiment, Microsoft Excel 2010 was used for data processing and analysis, and SPSS19.0 statistical software was used for data analysis of variance. Results Trehalose (10-20 mmol/L) significantly reduced the damage of NaCl to licorice seedlings, and the effect was the best when the concentration of exogenous trehalose was 15 mmol/L. Under NaCl stress, when exogenous trehalose concentration was 15 mmol/L, the growth of licorice seedlings was the most exuberant and the growth amount increased the most, and the K⁺ and K⁺/Na⁺ concentrations that affected the osmotic regulation of licorice seedlings increased the most, while the concentrations of Na⁺ and Cl^- were decreased compared with those without NaCl stress. When the concentration of exogenous trehalose was 15 mmol/L, the activity of antioxidant enzymes in licorice seedlings under NaCl stress could be improved, and the content of light and chlorophyll in plant cells under NaCl stress could be increased. When the concentration of exogenous trehalose was 15 mmol/L, the contents of soluble sugar, proline and cellular regulatory substance MDA in licorice seedlings under NaCl stress could be reduced. Conclusion The application of exogenous trehalose with appropriate concentration under NaCl stress can promote the growth of licorice seedlings and the accumulation of effective components, reduce the harm of salt damage to the growth of licorice, and enhance the growth ability of Licorice under NaCl stress.

R282.6

道地药材安全生产及绿色加工（2016C-15）