目的 对绿原酸及其13种体内主要代谢物的体外抗菌作用进行比较研究。方法 选择临床分离的常见致病菌如甲氧西林敏感金黄色葡萄球菌（MSSA）、耐甲氧西林金黄色葡萄球菌（MRSA）、β-内酰胺酶阴性大肠杆菌（E-）、产超广谱β-内酰胺酶大肠杆菌（E+）、肺炎克雷伯菌（E-/E+）、鲍曼不动杆菌和铜绿假单胞菌等，采用微量肉汤稀释法测定绿原酸代谢物的最低抑菌浓度，并与绿原酸进行比较。结果 对大多数受试菌株，部分主要代谢物抗菌作用与绿原酸比较有所增强，可提高4～66倍，以苯甲酸、对羟基苯甲酸、对香豆酸、二氢咖啡酸和没食子酸最佳，其中二氢咖啡酸、没食子酸对MSSA和MRSA的抗菌作用提高了33～66倍。结论 与绿原酸相比，绿原酸部分主要代谢产物抗菌活性增强，绿原酸进入体内可能通过其某些代谢产物而发挥抗菌作用。

Objective To compare with the antimicrobial activities of chlorogenic acid and its 13 main metabolites in vivo. Methods Main metabolites were detected by broth microdilution method against clinical isolated strains including methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), non ESBLs-producting Escherichia coli (E-), extended-spectrum β-lactamase Escherichia coli (E+), Klebsiella pneumoniae (E-/E+), Acinetobacter baumannii, and Pseudomonas aeruginosa to compare with the minimal inhibitory concentrations value of chlorogenic acid. Results For the most tested strains, the antibacterial activities of some major metabolites were enhanced, ranging from four to 66 times higher than chlorogenic acid. Benzoic acid, p-hydroxybenzoic acid, p-coumaric acid, dihydrocaffeic acid, and gallic acid were stronger than the other metabolites. Moreover, the antibacterial actions of dihydrocaffeic acid and gallic acid against MSSA and MRSA was increased 33-66 times respectively. Conclusion Some main metabolites presented more potent antibacterial activities than chlorogenic acid, and chlorogenic acid may exert antibacterial effects through some of its metabolites.

R285.5

国家自然科学基金资助项目（81803812）；四川省科技计划项目（2016JY0077）