目的 采用HPLC-DAD-ELSD及Box-Behnken响应面法优选黄芪Astragalus membranaceus var.mongholicus药材浸润工艺。方法 基于HPLC-DAD-ELSD及响应面设计方法，以饮片合格率、指标成分含量、弯折检查为综合考察指标，选取浸润温度、浸润时间、加水量3个因素进行响应面实验设计，优化黄芪药材浸润工艺参数。结果 最优浸润工艺为浸润温度20℃、浸润时间6 h，加水量为1∶0.988，黄芪饮片合格率为95.81%，毛蕊异黄酮苷为0.072%，黄芪甲苷为0.276%。结合指纹图谱及热图分析，黄芪药材在浸润过程中发生物质基础变化，浸润过程中应当严格控制浸润参数，保证饮片的质量均一。结论 优选后的黄芪药材浸润工艺稳定可行，重复性好，可以为黄芪药材饮片大批量生产工艺开发提供参考。

Objective To optimize the infiltration process of Astragalus (Astragalus membranaceus var. mongholicus) medicinal materials by Box-Behnken response surface method. Methods Based on the HPLC-DAD-ELSD and response surface design method, the qualified rate of decoction pieces, the content of index components and bending inspection were used as comprehensive inspection indicators, and the three factors of infiltration were selected for response surface experimental design to optimize the infiltration process of Astragalus medicinal materials parameter. Results The best infiltration process was as following:infiltration temperature was 20℃, with water addition of 1:0.988 for 6 h. Under this process, the qualified rate of Astragalus pieces was 95.81%, the content of calycosin-7-glucoside was 0.072%, and the content of astragaloside IV was 0.276%. Combining fingerprint analysis and heat map analysis, the material basis of A. membranaceus var. mongholicus changed during the infiltration process. The infiltration parameters should be strictly controlled during the infiltration process to ensure uniform quality of the pieces. Conclusion The optimized Astragalus medicinal material infiltration process is stable and feasible with good reproducibility, which can provide a reference for the mass production process development of Astragalus medicinal slices.

R283.1

国家科技重大专项课题（2017ZX09301005）