目的 制备桑色素-Cu²⁺配位分子印迹聚合物，对桑枝中桑色素进行分离富集。方法 在Cu²⁺存在下，丙烯酰胺为功能单体，在水-甲醇极性溶剂中制备桑色素-Cu²⁺配位分子印迹聚合物，以该聚合物为填料固相萃取桑枝中桑色素。结果 所制备配位分子印迹聚合物对桑色素具有较强的特异性、选择性吸附，最大吸附量82 μmol/g，远大于以氢键为作用力的传统印迹聚合物及非印迹聚合物；对桑色素结构类似物大豆素、儿茶素的分离因子分别为4.81、4.02。对比桑枝固相萃取淋洗液、洗脱液组成，所制备配位印迹聚合物对桑枝中桑色素表现出明显的富集效果。结论 制备的桑色素配位分子印迹聚合物环境适应性好，选择性吸附能力强，是适用于天然产物有效成分桑色素分离富集的良好材料。

Objective To prepare morin-Cu²⁺ complex imprinted polymer (CIP) and use the polymer to extract morin from mulberry branch. Methods Morin-Cu²⁺ CIPs were prepared in water-methanol polar solvent in the presence of Cu²⁺, acrylamide as the functional monomer. The CIPs were used as the solid phase extraction sorbent for extracting morin from mulberry branch. Results The prepared CIPs had specific and selective adsorption on morin, the maximum adsorption capacity was 82 μmol/g, which was much higher than that of the traditional imprinted polymers relying on hydrogen bond and non-imprinted polymers. The separation factors of CIPs to daidzein and catechin were 4.81 and 4.02, respectively. CIPs had a significant enrichment effect on morin in the composition of the mulberry branch solid phase extraction elution solution. Conclusion Morin-Cu²⁺ CIPs are excellent material used for the separation and enrichment of morin, the material has good selective adsorption ability and environmental adaptability.

R283.6

国家自然科学基金资助项目（31701042）；淄博市校城融合发展计划项目（2018ZBXC403）；淄博市校城融合发展计划项目开放课题（2019GECA018）；淄博市校城融合发展计划项目开放课题（2019GECA019）