目的 探究复方苦参注射液对膀胱癌T24细胞增殖、凋亡及自噬的影响，并探究其可能的分子机制，为复方苦参注射液在临床上治疗膀胱癌提供理论依据。方法 CCK-8法检测复方苦参注射液对T24细胞增殖的影响。流式细胞术检测复方苦参注射液对T24细胞凋亡的影响。MDC染色法检测复方苦参注射液对T24细胞自噬的影响。蛋白免疫印迹和免疫荧光实验检测复方苦参注射液对T24细胞凋亡关键蛋白和自噬关键蛋白的影响。结果 复方苦参注射液能有效抑制T24细胞增殖。复方苦参注射液能诱导T24细胞发生凋亡和自噬。蛋白免疫印迹和免疫荧光实验结果表明，随着复方苦参注射液剂量的升高，T24细胞中B细胞淋巴瘤2（Bcl-2）、自噬选择性底物（P62）的表达逐渐下降，B细胞淋巴瘤2相关X蛋白（Bax）、活化的天冬氨酸特异性半胱氨酸蛋白酶（cleaved-Caspase 3）、自噬关键分子酵母Atg6同系物（Beclin1）、微管相关蛋白B轻链3-Ⅱ（LC3Ⅱ）的表达逐渐升高（P<0.05），Bcl-2/Bax比值逐渐减小（P<0.05）。结论 复方苦参注射液能抑制T24细胞的增殖、调节T24细胞自噬，诱导T24细胞凋亡。

Objective To investigate the effect of Compound Kushen Injection on proliferation, apoptosis, and autophagy of bladder cancer T24 cells, and to explore the molecular mechanisms involved, and provide theoretical basis for Compound Kushen Injection in clinical treatment of bladder cancer. Methods The effect of Compound Kushen Injection on proliferation of bladder cancer cells was detected by CCK-8 method. Flow cytometry was used to detect the effect of Compound Kushen Injection on bladder cancer cell apoptosis. MDC staining was used to detect the effect of Compound Kushen Injection on autophagy of bladder cancer cells. Western blotting and immunofluorescence experiments were used to detect the effect of Compound Kushen Injection on key protein involved in apoptosis and autophagy of bladder cancer cells. Results Compound Kushen Injection effectively inhibited the proliferation of bladder cancer T24 cells. Compound Kushen Injection induced bladder cancer cells to undergo apoptosis and autophagy. The results of Western blotting and immunofluorescence experiments showed that as the concentration of Compound Kushen Injection increased, the expressions of Bcl-2 and P62 in bladder cancer T24 cells were gradually decreased, and the expressions of Bax, cleaved-Caspase 3, Beclin1, and LC3Ⅱ were increased (P<0.05), but Bcl-2/Bax ratio was decreased (P<0.05). Conclusion Compound Kushen Injection can inhibit the proliferation of bladder cancer T24 cells, regulate autophagy of bladder cancer T24 cells, and induce bladder cancer T24 cell apoptosis.

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河北省自然科学基金资助项目（H2018201179）；河北省教育厅青年基金资助项目（QN2019146）；河北省卫生厅科研基金资助项目（20190948）；河北大学医学学科培育项目（2020A15）；国家大学生创新创业项目（2020376）