目的 制备积雪草苷-海藻酸钠修复贴（As-SA RP）并评价其对皮肤创伤修复的效果。方法 利用海藻酸钠与Ca²⁺交联成膜的特点，以积雪草苷为药物模型，通过与海藻酸钠共混的方式成功制备了As-SA RP，采用扫描电子显微镜及红外光谱考察共混膜的相容性，并用新西兰大耳兔背部脊柱两侧的全层皮肤建立创伤模型，通过观察各组家兔受损皮肤的愈合状况并测定其受损皮肤组织中胶原（羟脯氨酸作为指标）和促炎症因子（TNF-α、IL-1β和IL-6）的表达水平以考察外敷As-SA RP对皮肤创面的修复效果。结果 积雪草苷与海藻酸钠具有良好的相容性，可成功制备As-SA RP修复贴。相较于其他组，As-SA RP处理后皮肤受损处的红肿、出血、感染、渗出液情况明显减少，创面愈合率也相对升高。病理结构显示As-SA RP组家兔皮肤中炎性细胞浸润较少、新生的毛细血管较多、创面皮肤结构较完整且胶原纤维排列相对紧密有序。同时，生化分析结果显示As-SA RP能有效抑制受损皮肤组织中促炎症因子TNF-α、IL-1β、IL-6的高表达，并可明显增高其中羟脯氨酸及胶原纤维含量。结论 As-SA RP可在一定程度上提升皮肤对积雪草苷的吸收，进而表现出比活性组分更好的皮肤损创伤修复效果。

Objective Asiaticoside-sodium alginate repair patch (As-SA RP) was prepared and its effect on skin wound healing was investigated. Methods Based on the cross-linking characteristics of sodium alginate and Ca²⁺, As-SA RP was prepared using asiaticoside as a drug model through blending with sodium alginate. The compatibility of the blend films was investigated by scanning electron microscope and FTIR. The whole layer of skin on both sides of the back spine of New Zealand's big ear rabbit was used to establish the trauma model. The repair effect of the external application of As-SA RP on skin wounds was investigated by observing the healing status and determining the expression levels of collagen (hydroxyproline as an index) and pro-inflammatory (TNF-α, IL-1β and IL-6) factors in the injured skin tissue. Results Asiaticoside had good compatibility with sodium alginate and can be successfully prepared As-SA RP. Compared with other groups, the red swelling, bleeding, infection, and exudate on injured skin were significantly reduced, and the wound healing rate was also relatively increased after As-SA RP treatment. Moreover, the less inflammatory cells infiltration, more new capillaries and intact wound skin structure relatively tight and orderly arrangement of collagen fibers were observed in the As-SA RP group. On the other hand, the biochemical analysis showed that As-SA RP could effectively inhibit the high expression of TNF-α, IL-1β, IL-6 in the injured skin tissue and significantly increase the contents of hydroxyproline and collagen fiber. Conclusion As-SA RP could improve the skin absorption of asiaticoside and show the better effect of skin wound healing than the active component asiaticoside.

R283.6

国家自然科学基金青年科学基金项目（81803684）；广东海洋大学大学生创新创业训练项目（CXXL2019290）；广东海洋大学本科生天然海洋药物创新团队（CCTD201828）