目的 研究滇重楼内生真菌米曲霉Aspergillus oryzae次生代谢产物的化学成分及结构。方法 将米曲霉采用液体发酵方式发酵，萃取后运用多种色谱方法进行分离纯化，并根据理化性质和波谱数据鉴定化合物的结构。结果 从滇重楼内生真菌米曲霉发酵产物中分离鉴定了4个化合物，分别鉴定为4-羟基-6-[（2S，3S）-3-羟基丁烷-2-基]-3-甲基-2H-吡喃-2-酮（1）、（R）-4-羟基-6-（1-羟基-2-甲基丙基）-3-甲基-2H-吡喃-2-酮（2）、flufuran（3）、flufuran methyl ester（4）。结论 化合物1、2为新的α-吡喃酮类化合物，命名为米曲霉α-吡喃酮A和米曲霉α-吡喃酮B。

Objective To study the chemical composition and structure of the secondary metabolites of the endophytic fungus Aspergillus oryzae from Paris polyphylla var. yunnanensis. Methods A. oryzae was fermented by liquid fermentation. After extraction, it was separated and purified by various chromatography methods. The structure of the compounds was identified according to the physical and chemical properties and spectral data. Results Four compounds were isolated and their structures were identified as 4-hydroxy-6-[(2S,3S)-3-hydroxybutan-2-yl]-3-methyl-2H-pyran-2-one (1), (R)-4-hydroxy-6-(1-hydroxy-2-methylpropyl)-3-methyl-2H-pyran-2-one (2), flufuran (3) and flufuran methyl ester (4). Conclusion Compounds 1 and 2 are new α-pyronoids named asper-α-pyranone A and asper-α-pyranone B.

R284.2;R286.02

国家自然科学基金项目（31560099）；国家自然科学基金项目（31860099）；云南民族大学大学生创新创业训练项目；云南民族大学化学学科博士点培育项目（BSD-HX-2019-20）