目的 基于网络药理学方法研究参芪降糖颗粒治疗糖尿病微循环障碍的潜在分子机制。方法 通过GeneCards数据库查询糖尿病肾脏病、糖尿病视网膜病变、糖尿病神经病变、糖尿病足病和糖尿病合并脑小血管病的疾病靶点，整合为糖尿病微循环障碍疾病靶点。借助PubChem Search和Swiss target prediction在线工具得到参芪降糖颗粒药效成分治疗糖尿病微循环障碍的作用靶标，利用Cytoscape 3.3.0软件构建参芪降糖颗粒化学成分-糖尿病微循环障碍作用靶标网络，通过STRING数据库构建靶蛋白相互作用（PPI）网络，利用Clue GO插件进行GO分析和KEGG通路富集分析。结果 从参芪降糖颗粒中共筛选出85个化学成分，以及10个与糖尿病微循环障碍相关的作用靶点，分别为ACE、VEGFA、TNF、IL6、STAT3、ALB、PON1、PTPN22、PPARG、NOS3。经GO和KEGG通路富集分析，发现参芪降糖颗粒治疗糖尿病微循环障碍可能参与HIF-1信号通路、胰岛素抵抗、AGE-RAGE信号通路、炎症性肠病、类风湿性关节炎、肥厚性心肌病信号通路等多条信号通路。结论 参芪降糖颗粒治疗糖尿病微循环障碍具有多成分-多靶点-多途径的作用特点，为进一步拓展参芪降糖颗粒的临床应用提供了借鉴和参考。

Objective To explore the mechanism of Shenqi Jiangtang Granules (SJG) in the treatment of diabetic microcirculation disorder based on network pharmacology. Methods The targets of diabetic kidney disease, diabetic retinopathy, diabetic peripheral neuropathy, diabetic foot disease and small cerebral vascular disease were searched through Genecards database to be integrated as the targets of diabetic microcirculation disorder disease. The targets of SJG' active components for treatment of diabetic microcirculation disorder were screened and predicted by utilizing PubChem Search and Swiss target prediction online tool. Cytoscape 3.3.0 software was adopted to construct an active component-diabetic microcirculation disorder target network. The protein-protein interaction (PPI) network was established by using STRING database. Gene ontology (GO) biological process and Kyoto Encyclopedia of Genes and Gnomes (KEGG) pathway enrichment analysis were performed using Clue GO. Results A total of 85 active components of SJG and 10 targets (ACE, VEGFA, TNF, IL6, STAT3, ALB, PON1, PTPN22, PPARG and NOS3) related to diabetic microcirculation disorder were screened. Through GO and KEGG pathway enrichment analysis, it was found that the active components of SJG in the treatment of diabetic microcirculation disorder may participate in multiple signaling pathways, such as AGE-RAGE signaling pathway in diabetic complications, HIF-1 signaling pathway, insulin resistance, hypertrophic cardiomyopathy, inflammatory bowel disease, rheumatoid arthritis, etc. Conclusion This study reflects the characteristics of multi-components, multi-targets and multi-pathways of SJG, which provides new ideas and clues for further research on the mechanism of SJG in the treatment of diabetic microcirculation disorder.

R285.5

国家重点研发计划项目（2018YFC1704200）；国家重点研发计划项目（2018YFC1704205）；国家自然科学基金重点项目（81530102）；国家自然科学基金重点项目（81830113）