目的 分离纯化乌拉尔甘草内生真菌，及筛选其次生代谢产物具有较好抑菌活性、抗氧化活性（DPPH·自由基清除率、总还原力测定、羟基自由基清除率）、抗α-葡萄糖苷酶活性的特异性菌株。方法 采用组织切块法对乌拉尔甘草分离纯化内生真菌；以正丁醇、醋酸乙酯和乙醇为有机相萃取菌株发酵液及菌丝体；采用滤纸片法进行抑菌活性检测；3种检测方法表征抗氧化活性；PNPG法检测抗α-糖苷酶活性。结果 从乌拉尔甘草中分离得到36株内生真菌，发酵液萃取浓缩得到108个样品。实验共分离得到7个属，其中木霉属是优势种。实验结果显示，43.52%的样品具有不同程度的抑菌作用，其中8.33%表现较好；萃取物均具有不同程度的抗氧化活性，其中4.63%~10.12%的样品分别在3种方法中表现较好；99.07%的样品具有不同程度的抗α-葡萄糖苷酶活性，其中5.56%的样品表现较好。结论 从乌拉尔甘草中分离出具备较好的抑菌活性、抗氧化活性、抗α-葡萄糖苷酶活性代谢产物的菌株，有待进一步研究深入。

Objective To isolate and purify endophytic fungus from Glycyrrhiza uralensis, and screen the specific strain with better antibacterial and antioxidant activity (DPPH· radical scavenging, total reducing power, determination of hydroxyl radical scavenging) and anti-alpha-glucosidase activity. Methods The endophytic fungi were isolated and purified from G. uralensis by tissue cutting. N-butanol, ethyl acetate and ethanol were used to extract the fermentation liquid and mycelium. Antibacterial activity was detected by filter paper. Three methods were used to characterize antioxidant activity. A total of 36 endophytic fungi were isolated from G. uralensis by PNPG method. Results A total of seven genera were isolated from 108 samples by concentrated fermentation liquid extraction. Trichoderma. sp was the dominant species. The experimental results showed that 43.52% of the samples had different degrees of antibacterial effect, of which 8.33% performed well; The extracts showed different levels of antioxidant activity, of which 4.63% to 10.12% showed better performance in the three methods. 99.07% of the samples had different levels of anti-α-glucosidase activity, of which 5.56% of the samples performed well. Conclusion The strains isolated from G. uralensis have good antibacterial activity, antioxidant activity, and anti-alpha-glucosidase activity, which further study is needed.

R282.2

国家自然科学基金资助项目（81660581）；国家自然科学基金资助项目（31460032）；兰州理工大学红柳优秀青年人才支持计划（2018）