目的 探讨小檗碱调节糖原代谢的机制及对糖原结构的影响。方法 采用自发型糖尿病模型db/db小鼠作为疾病模型鼠，考察小檗碱对db/db小鼠血糖水平的影响；提取小鼠的肝糖原进行体积排阻色谱（SEC）和透射电镜（TEM）分析，考察小檗碱对db/db小鼠肝糖原的结构影响，并探讨其影响机制。结果 小檗碱可以显著性降低db/db小鼠的空腹血糖，降低db/db小鼠血清胰岛素水平；改善db/db小鼠肝糖原的结构不稳定性；降低db/db小鼠肝组织中糖原磷酸化酶（GP）、糖原脱分支酶（GDBE）和环磷酸腺苷（cAMP）表达水平，降低血清胰高血糖素水平。结论 小檗碱可以调控cAMP/GP信号通路，改善db/db小鼠的肝糖原结构，修复受损糖原结构，这可能是其调节肝糖代谢，改善糖尿病症状的机制之一。

Objective To investigate the mechanism of berberine regulating glycogen metabolism and its effect on glycogen structure. Methods The db/db mice were used as diabetic mice to investigate the effect of berberine on blood glucose levels in db/db mice. The liver glycogen was extracted for SEC and TEM analysis to investigate the effect of berberine on liver glycogen and the mechanism of its effect. Results Berberine significantly decreased the fasting blood glucose level and the insulin level in serum of db/db mice, and berberine repaired the damaged glycogen structure. Meanwhile, berberine decreased the expression of GP, GDBE, cAMP in liver tissue and the glucagon level in serum of db/db mice. Conclusion Berberine regulated the cAMP/GP signaling pathway, improved hepatic glycogen structure in db/db mice, and repaired damaged glycogen structure, which may be one of the mechanisms for regulating hepatic glucose metabolism and improving symptoms of diabetes.

R285.5

国家自然科学基金资助项目（81803799）；中央高校基本科研业务费专项资金资助项目（2017KFYXJJ252）