目的 探讨番茄红素对肥胖小鼠糖脂代谢和胰腺组织炎症的影响及其可能的作用机制。方法 利用高脂饲料复制肥胖小鼠模型。番茄红素干预8周后，观察其对小鼠体质量、血糖、血脂和体脂率的影响。采用HE染色法观察胰腺病理改变，Western blotting法检测Toll样受体4（TLR4）/髓样分化因子88（MyD88）/核转录因子-κB（NF-κB）信号通路相关蛋白表达水平，免疫组化法检测各组小鼠胰腺组织巨噬细胞浸润程度。结果 番茄红素能显著抑制高脂饮食诱导的肥胖小鼠体质量增长，降低空腹血糖，改善糖耐量及血脂水平；减轻胰腺组织的空泡化、水肿变性以及胰岛肥大等炎性病变；同时，番茄红素可明显降低TLR4、MyD88和NF-κB蛋白在胰腺组织中的表达水平，减轻胰腺炎性浸润。结论 番茄红素能够改善高脂饮食诱导肥胖小鼠的血糖、血脂水平和胰腺炎症，其作用机制可能与调控TLR4/MyD88/NF-κB信号通路有关。

Objective To investigate the effects of lycopene on glycolipid metabolism and pancreatic tissue inflammation in obese mice and its underlying mechanism. Methods The obese mouse model was induced by a high-fat diet (HFD). The effects of lycopene on body weight, blood glucose, blood lipid and body fat were observed after 8 weeks of administration. Pathological changes of pancreas were observed by HE staining. Protein expressions involved in TLR4/MyD88/NF-κB signaling pathway were detected by Western blotting, and the degree of macrophage infiltration in the pancreatic tissues of obese mice were detected by IHC. Results Lycopene significantly inhibited body weight gain and reduced fasting blood glucose, as well as improved glucose tolerance and blood lipid level in obese mice. In addition, lycopene also reduced vacuolization, edema degeneration, islet hypertrophy and other inflammatory lesions in pancreatic tissues. Moreover, the protein expression levels of TLR4, MyD88 and NF-κB in pancreatic tissue were decreased and the inflammatory infiltration was reduced. Conclusion Lycopene can improve blood glucose, lipid metabolism and pancreatic inflammation in obese mice, and its mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway.

R285.5

国家自然科学基金面上项目（81874373）；北京市自然科学基金面上项目（7172126）