目的 采用脂多糖（LPS）诱导RAW264.7细胞的炎症模型，考察炎症相关指标，探讨毛蕊花苷的抗炎作用及其机制。方法 用MTT比色法检测毛蕊花苷对RAW264.7细胞活性的影响；Griess法检测NO的含量；Western blotting检测核转录因子-κB（NF-κB）通路相关蛋白的表达，ELISA试剂盒检测肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的释放，流式细胞仪检测细胞内活性氧（ROS）、一氧化氮（NO）、Ca²⁺的释放情况。结果 毛蕊花苷浓度为20、40、80 μmol/L时对RAW264.7细胞没有毒性。不同浓度的毛蕊花苷对LPS诱导RAW264.7细胞导致的NO、TNF-α、IL-6的释放，细胞内ROS、NO、Ca²⁺释放的增加均有很好的抑制效果，对诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、p-IKKα/β、p-p65、p-IκBα蛋白的表达也有抑制作用。结论 毛蕊花苷具有明显的抗炎作用，其作用机制是通过抑制NF-κB信号通路而发挥抗炎作用。

Objective To investigate the anti-inflammatory effects and mechanism of verbascoside in LPS-stimulated RAW264.7 cells. Methods MTT assay was used to determine cytotoxicity of verbascoside in RAW264.7 cells. Griess assay was employed to detect nitrite level in LPS-stimulated RAW264.7 cells. The protein expressions of iNOS, COX-2, p-IKKα/β, p-p65, and p-IκBα were detected by Western blotting. The release of TNF-α and IL-6 was detected by ELISA. The levels of intracellular ROS, NO, and Ca²⁺ were detected by a flow cytometry. Results Mullein glycoside (20, 40, and 80 μmol/L) displayed no significant cytotoxicity in RAW264.7 cells. The nitrite level, TNF-α and IL-6 release, and intracellular ROS, NO, and Ca²⁺ level were significantly decreased by Mullein glycoside (20, 40, and 80 μmol/L). In addition, verbascoside also inhibited the protein expressions of iNOS, COX-2, p-IKKα/β, p-p65, and p-IκBα. Conclusion Verbascoside showed an obvious anti-inflammatory effect via the NF-κB signaling pathway.

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国家青年科学自然基金项目（81803807）；广西自治区青年科学基金项目（2018JJB140265）；广西高校引进海外高层次人才“百人计划”项目（05018064）；广西科技基地和人才专项（2018AD19034）