目的 对桂枝茯苓胶囊内容物正丁醇萃取部位进行化学成分研究和活性筛选。方法 采用多种色谱方法进行分离纯化，应用波谱数据解析鉴定化合物结构；对分离得到化合物进行原代小鼠子宫平滑肌细胞的钙离子内流作用的活性筛选。结果 从桂枝茯苓胶囊内容物正丁醇层分离得到5个没食子酰苷类化合物，分别鉴定为3'-O-没食子酰蔗糖苷（1）、4'-O-没食子酰蔗糖苷（2）、6'-O-没食子酰蔗糖苷（3）、1'-O-没食子酰蔗糖苷（4）、1，2，3，4，6-五没食子酰葡萄糖（5）。结论 化合物1～5为首次从该复方中分离得到，其中化合物1为新化合物；化合物1～5均有抑制原代小鼠子宫平滑肌细胞的钙离子内流作用。

Objective To study the chemical constituents from the n-butanol extract of Guizhi Fuling Capsule. Methods The chemical constituents were isolated and purified by multiple chromatographic methods. Their structures were identified on the basis of the spectral data and physicochemical properties. Results Five compounds were identified as 3'-O-galloylsucrose (1), 4'-O-galloylsucrose (2), 6'-O-galloylsucrose (3), 1'-O-galloylsucrose (4), and 1,2,3,4,6-pentagalloylglucose (5). Conclusion Compounds 1-5 are isolated from Guizhi Fuling Capsule for the first time, and compound 1 is a new compound. Compounds 1-5 inhibited calcium influx in uterine smooth muscle cells of primary mice.

R284.1

国家中药标准化专项：桂枝茯苓胶囊标准化建设（ZYBZH-C-JS-28）；国家重大新药创制科技重大专项：基于功效成分群的中药口服固体制剂先进制药与信息化技术融合示范应用（2018ZX09201010-004）