目的 建立HPLC法测定12个不同产地黄秋葵中槲皮素-3-O-龙胆二糖苷、槲皮素-3-O-芸香糖苷、异槲皮苷、槲皮素、5，7，4'-三羟基黄酮的含量，为其合理开发利用提供参考。方法 采用COSMOSIL色谱柱（250 mm×4.6 mm，5.4 μm）；以乙腈和0.2%的磷酸水溶液为流动性进行梯度洗脱，检测波长为353 nm，体积流量1 mL/min。结果 槲皮素-3-O-龙胆二糖苷、槲皮素-3-O-芸香糖苷、异槲皮苷、槲皮素、5，7，4'-三羟基黄酮分别在15.72～251.50 μg/mL（r=0.999 5）、15.47～247.50 μg/mL（r=0.999 6）、14.41～230.50 μg/mL（r=0.999 5）、21.88～350.00 μg/mL（r=0.999 7）、17.25～276.00 μg/mL（r=0.999 1）浓度范围内呈良好的线性关系；平均加样回收率分别为97.46%、96.74%、100.21%、95.66%、98.35%，RSD为1.97%、1.37%、1.86%、2.72%、2.23%。结论 方法简单易行、准确度高，适合于同时测定槲皮素-3-O-龙胆二糖苷、槲皮素-3-O-芸香糖苷、异槲皮苷、槲皮素、5，7，4'-三羟基黄酮的含量，可作为黄秋葵质量评价的方法之一。

Objective To establish a method for determination of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin, and 5,7,4'-trihydroxyflavone in Abelmoschus esculentus from 12 different regions of China. Methods COSMOSIL column (250 mm×4.6 mm, 5.4 μm) was used. The gradient elution was carried out with acetonitrile and 0.2% phosphoric acid aqueous solution. The detection wavelength was 353 nm and the flow rate was 1 mL/min. Results The contents of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin and 5,7,4'-trihydroxyflavone had good linear relationship in the ranges of 15.72- 251.50 μg/mL (r=0.999 5), 15.47-247.50 μg/mL (r=0.999 6), 14.41-230.50 μg/mL (r=0.999 5), 21.88-350.00 μg/mL (r=0.999 7), and 17.25-276.00 μg/mL (r=0.999 1). The average sample recovery rate was 97.46%, 96.74%, 100.21%, 95.66%, 98.35%, and RSD was 1.97%, 1.37%, 1.86%, 2.72%, 2.23%, respectively. Conclusion This method is simple and accurate. It is suitable for simultaneous determination of quercetin-3-O-gentiobioside, quercetin-3-O-rutinoside, isoquercitrin, quercetin and 5,7,4'-trihydroxyflavone. The content of the five flavones can be used as one of the methods for evaluating the quality of A. esculentus.

R286.2

江西省教育厅科学技术研究项目（GJJ151024）；江西省教育厅科学技术研究项目（GJJ161040）