目的 建立以HPLC法同时检测16种33批五加属植物中紫丁香苷、绿原酸、咖啡酸、对香豆酸、阿魏酸、ethylsyringin、ethylconiferin、（+）-梣皮树脂醇、（±）-松脂素、（±）-丁香脂素、taiwanin C、savinin、helioxanthin、（-）-sesamin 14种苯丙素类成分的含量。方法 采用Promosil C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，体积流量1 mL/min；柱温30℃；检测波长280 nm。结果 在优化的色谱条件下，14种成分在测定的质量浓度范围内线性关系良好，r均大于0.999 1，方法的精密度、稳定性、重复性以及回收率良好。结论 建立的HPLC法可用于同时检测五加属植物中14种苯丙素类化学成分的含量，该方法快速、准确，有较好的重现性和稳定性，可为该属植物的进一步研究提供参考。

Objective To establish an HPLC method for the simultaneous determination of syringin, chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, ethylsyringin, ethylconiferin, (+)-fraxinol, (±)-rosin, (±)-syringin, taiwanin C, savinin, helioxanthin, and (-)-sesamin in 16 species and 33 batches of Acanthopanax plants. Methods Separation was carried out on Promosil C₁₈ column (250 mm×4.6 mm, 5 μm), the mobile phase was eluted with acetonitrile-0.1% phosphoric acid. The flow rate was 1 mL/min, and the column temperature was 30 ℃, the detection wavelength was 280 nm. Results Under the optimized chromatographic conditions, the linear relationship of the 14 constituents was good in the range of mass concentration, r was more than 0.999 1, with good precision, stability, repeatability and recovery. Conclusion HPLC method established in this study is effective, accurate, and reproducible and can be used for the simultaneous determination of phenylpropanoids in Acanthopanax plants, which can provide reference for the further study of Acanthopanax plants.

R286.2

湖南省自然科学基金项目（2019JJ40223）；湖南省自然科学基金项目（2019JJ50448）；湖南省教育厅一般项目（18C0399）；湖南中医药大学生物工程重点学科资助（校行科字[2018]3号）