目的 探索三七活血止血、消肿止痛传统功效的网络调控机制。方法 选取三七药材中12个入血成分为研究对象，依据反向分子对接的方法预测化合物靶点，借助String 10数据库与Omicsbean在线分析软件对靶点进行信号通路分析、基因本体（gene ontology，GO）功能富集分析，利用Cytoscape软件构建网络。结果 12个化合物（三七皂苷R₁、人参皂苷Rg₁、人参皂苷Re、人参皂苷Rh₁、人参皂苷Rg₂、人参皂苷Rb₁、人参皂苷Rd、人参皂苷F₂、人参皂苷Rg₃、人参皂苷Rk₁、三七素、槲皮素）可通过65个相关靶点作用于65条信号通路，主要涉及抑制血栓生成、纤溶、血管新生、舒张血管、凝血、抗炎以及镇痛等方面，进一步得到三七“化合物-靶点-通路-药理作用-功效”的网络药理图。结论 三七通过作用于F2、F10、PLAT、VEGFA、NOS2、IL6、PTGES、OPRD1等关键蛋白干预了多个与活血止血、消肿止痛相关的生物过程，初步揭示了其传统功效的作用机制。

Objective To explore the network regulation mechanism of blood-activating and hemostatic and detumescent and analgesic traditional effects of Panax notoginseng. Methods Targets of the 12 components of P. notoginseng absorbed in plasma were predicted according to the reverse pharmacophore method. Gene ontology (GO) function enrichment and pathway analysis of the targets were analyzed by Omicsbean online analysis software and String 10 database. Finally, Cytoscape software was used to construct the network pharmacology map. Results A total of 12 compounds (notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rh₁, ginsenoside Rg₂, ginsenoside Rb₁, ginsenoside Rd, ginsenoside F₂, ginsenoside Rg₃, ginsenoside Rk₁, dencichine and quercetin) affected 65 pathways through 65 related targets, which were associated with anti-thrombosis, fibrinolysis, angiogenesis, vasodilation, blood coagulation, anti-inflammation and analgesia. The network of “compound-target-pathway-pharmacological action-efficacy” was also constructed. Conclusion P. notoginseng interferes with multiple biological processes related to activating blood circulation, hemostasis, detumescence and analgesia by acting on several key proteins such as F2, F10, PLAT, VEGFA, NOS2, IL6, PTGES, OPRD1, etc.

R285.5

国家自然科学基金重点项目（81830111）；天津市自然科学基金项目（19JCQNJC12700）