目的 建立黄芪饮片、标准汤剂、配方颗粒的HPLC特征图谱，对其相关性进行评价。方法 采用HPLC法测定黄芪饮片、标准汤剂、配方颗粒中7-羟基-4'-甲氧基异黄酮、毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、芒柄花苷含量，以HPLC特征图谱为对照，建立三者指纹图谱共有模式，并进行相关性评价。结果 20批黄芪饮片中7-羟基-4'-甲氧基异黄酮、毛蕊异黄酮葡萄糖苷、毛蕊异黄酮、芒柄花苷平均质量分数分别为0.097、0.482、0.142、0.237 mg/g；标准汤剂中这4种指标成分的平均质量分数分别为0.116、0.912、0.214、0.434 mg/g；配方颗粒中这4种指标成分的平均质量分数分别为0.088、0.623、0.160、0.289 mg/g，20批黄芪饮片、标准汤剂、配方颗粒指纹图谱均呈现6个共有特征峰，黄芪配方颗粒与标准汤剂对照指纹图谱相似度为0.980，说明两者的成分差异很小。结论 建立黄芪饮片、标准汤剂、配方颗粒的指纹图谱，全面反映黄芪饮片-标准汤剂-配方颗粒的多组分情况，为黄芪配方颗粒的鉴定及质量控制提供参考价值。

Objective To establish an HPLC fingerprint spectrum of for decoction pieces-, standard decoction- and dispensing granules of Astragali Radix, and evaluate the correlation among them. Methods HPLC method was used to determine the content of 7-hydroxy-4'-methoxy isoflavone, calycosin-7-glucoside, calycosin, and ononin in decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. The common pattern of the three fingerprints was established and the correlation was evaluated through the control fingerprint spectrum. Results The average contents of 7-hydroxy-4'-methoxy isoflavone, calycosin-7-glucoside, calycosin, and ononin in 20 batches of decoction pieces were 0.097, 0.482, 0.142, and 0.237 mg/g, respectively; The average content of these four index components in the standard decoction were 0.116, 0.912, 0.214, and 0.434 mg/g, respectively; The average content of these four index components in the dispensing granules were 0.088、, 0.623、, 0.160、and 0.289 mg/g, respectively. There were six common peaks in all fingerprint spectra of 20 batches of decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. The similarity between dispensing granules and standard decoction was 0.980, indicating that the components difference between them was very small. Conclusion The study establishes an HPLC fingerprint spectrum, and itwhich reflects the whole picture of multi-component of decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. It provides reference value for the identification and quality control of Astragalus dispensing granules of Astragalus Radix.

R286.02

甘肃省中医药管理局科研项目（GZK-2019-41）；甘肃省人民医院研发攻关项目（18GSSY2-3）