目的 通过对枳实进行多成分定性、定量分析，为不同产地酸橙枳实药材的质量控制提供依据。方法 结合指纹图谱和一测多评（QAMS）模式，采用Waters X-Brige C₁₈（250 mm×4.6 mm，5 μm）色谱柱，以乙腈（A）-0.1%甲酸水溶液（B）为流动性，梯度洗脱，检测波长为283 nm和330 nm，体积流量1 mL/min，柱温30℃，进样量10 μL。结果 建立了不同产地酸橙枳实药材的HPLC-UV指纹图谱，确定了21个共有峰，鉴别出了芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、柚皮素、橙皮素、甜橙黄酮、川陈皮素、桔皮素9种成分，且23批次枳实样品的相似度均大于0.9，聚类分析（CA）及主成分（PCA）分析结果显示的分类情况与该药材的产地分布情况相一致，以新橙皮苷为参照物的QAMS法测定结果均显示一定的差异性。结论 从成分的角度来看，不同产地间药材质量存在一定差异，实验所建的QAMS法准确、高效、可行，与标准曲线法和外标法相比具有明显的优势，可应用于酸橙枳实的质量评价，为不同产地酸橙枳实药材的质量控制奠定实验基础。

Objective This paper aims to provide the experimental foundation for quality control of Aurantii Immaturus Fructus from different habitats by analyzing the multi-component qualitatively and quantitatively. Methods Fingerprint pattern and QAMS were combined in this experiment, and HPLC analysis was performed on a Waters X-Brige C₁₈ (200 mm×4.6 mm, 5 μm) column with mobile phase composed of acetonitrile (A) -0.1% methanoic acid solution at a flow rate of 1.0 mL/min in gradient elution mode. The column temperature was maintained at 30 ℃, the injection volume was 10 μL, and the detection wavelengths were set at 283 and 330 nm. Results The HPLC-UV fingerprint of Aurantii Immaturus Fructus from different habitats was established with 21 common peaks, 9 of them were identified as narirutin, naringin, hesperidin, neohesperidin, naringenin, hesperetin, sinensetin, nobiletin and tangeretin, and the similarity of 23 batches of samples were greater than 0.9, CA and PCA show the classification is consistent with the habitats distribution of the medicine. Then, the QAMS result showed some differences with neohesperidin to be the internal standard. Conclusion From the perspective of composition, there are some differences in the quality of Aurantii Immaturus Fructus from different habitats, and the method of QAMS is accurate, efficient and feasible, which has obvious advantages than standard curve method and external standard method. In a word, it can be applied to quality evaluation and provides the experimental foundation for quality control of Aurantii Immaturus Fructus from different habitats.

R286.2

江苏省研究生科研与实践创新计划项目（KYCX19_1265）；江苏省高等学校自然科学研究项目（19KJB360004）