目的 研究芸香科黄皮属植物光滑黄皮Clausena lenis茎中的化学成分。方法 综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离和纯化，根据化合物的理化性质及其波谱数据，并通过与文献中报道的波谱数据进行对照，鉴定化合物的化学结构。结果 从光滑黄皮茎的90%乙醇提取物中分离得到了18个化合物，分别鉴定为6，8-diprenylumbelliferone（1）、byakangelicin（2）、tert-O-methylheraclenol（3）、pabularinone（4）、异栓翅芹烯醇（5）、独活属醇（6）、欧前胡素（7）、前胡醇（8）、花椒树皮素甲（9）、异紫花前胡内酯（10）、茵芋碱（11）、香草木宁（12）、N-metilatanina（13）、clausine Z（14）、九里香碱（15）、2-methoxy-1-（3-methyl-buten-1-yl）-9H-carbazole-3-carbaldehyde（16）、claulansine I（17）和clausehainanine A（18）。其中化合物1～10为香豆素类化合物，11～18为生物碱类化合物。结论 所有化合物均为首次从光滑黄皮中分离得到，其中化合物2、3和8为首次从黄皮属植物中分离得到。

Objective To study the coumarins and alkaloids from the stems of Clausena lenis. Methods The chemical constituents from the stems of C. lenis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as the comparisons with the data reported in literatures. Results A total of 18 compounds were isolated from the 90% ethanol extract of the stems of C. lenis, which were identified as 6,8-diprenylumbelliferone (1), byakangelicin (2), tert-O-methylheraclenol (3), pabularinone (4), isogosferol (5), heraclenol (6), imperatorin (7), decursinol (8), xanthyletin (9), marmesin (10), skimmianine (11), kokusaginine (12), N-metilatanina (13), clausine Z (14), murrayanine (15), 2-methoxy-1-(3-methyl-buten-1-yl)-9H-carbazole-3-carbaldehyde (16), claulansine I (17) and clausehainanine A (18). Among them, compounds 1-10 were coumarins and 11-18 were alkaloids. Conclusion All compounds are isolated from C. lenis for the first time, compounds 2, 3 and 8 are separated from the genus Clausena for the first time.

R284.1

国家自然科学基金资助项目（31660097）；国家自然科学基金资助项目（21662011）；国家自然科学基金资助项目（21967008）；海南省重点研发计划项目（ZDYF2019049）；海口市重点研发计划项目（2017050）；教育部创新团队项目（IRT-16R19）