[关键词]
[摘要]
目的 研究不同产地野生远志Polygala tenuifolia中5种主要成分的含量差异,为远志多成分质量评价体系提供基础。方法 采用HPLC法测定20个产地野生远志中远志(口山)酮III、3,6'-二芥子酰基蔗糖、远志酸、远志皂苷元和细叶远志皂苷的含量,运用SPSS 20.0和SIMCA 11.5进行差异性、聚类、相关性和主成分分析。结果 不同产地野生远志中5种主要成分的含量差异显著,聚类分析和主成分分析可将所有样品聚为2类,第I类所含3,6'-二芥子酰基蔗糖、远志酮III、远志皂苷元和细叶远志皂苷含量较高,来自陕西渭南、陕西咸阳和山西新绛,其余样品则聚为第II类。结论 陕西渭南、陕西咸阳和山西新绛所产野生远志中主要成分含量较高,与其他产地存在极显著性差异,可为远志质量评价、优良种质及栽培基地选择等提供依据。
[Key word]
[Abstract]
Objective The contents of five main components in Polygala tenuifolia from different habitats were determined, which provided certain data support and theoretical basis for the quality evaluation system of P. tenuifolia. Methods The contents of polygalaxanthone III, 3,6'-disinapoyl sucrose, polygalacic acid, senegenin and tenuifolin of roots from different wild samples were determined by HPLC. Then SPSS 20.0 and SIMCA 11.5 were used for difference analysis, correlation analysis, hierarchical cluster analysis (HCA), and principal component analysis (PCA). Results The content of the five main components in wild P. tenuifolia samples from different habitats was significantly different. The 20 samples were placed into two clusters (I, II) by HCA and PCA. Cluster I comprised three samples with higher content of 3,6'-disinapoyl sucrose, polygalaxanthone III, senegenin and tenuifolin from Weinan, Xianyang in Shannxi Province, and Xinjiang in Shanxi Province, whereas cluster II contained the other 17 samples. Conclusion The results showed that the main components of P. enuifolia from Weinan, Xianyang in Shannxi Province and Xinjiang in Shanxi Province were significantly higher than other origins, and which provided a reference for the quality control, selection of excellent germplasm and cultivation bases of P. tenuifolia.
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[基金项目]
公益性行业(中医药)科研专项经费项目(201507002-1-08);陕西中医药大学"秦药"品质评价及资源开发学科创新团队项目(2019-QN01);2019年医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)"全国中药资源普查项目"(财社[2019]39号);2018全国中药特色技术传承人才(20184828005)