目的 客观评价三七内在质量，构建三七质量评价的灰色关联模型，为三七质量评价及多指标成分中药材质量评价提供参考。方法 采用HPLC法同时测定不同产地、不同商品规格三七样品中三七皂苷R₁，人参皂苷Rg₁、Re、Rb₁、Rd含量，结合灰色关联法建立整体质量评价模型。结果 所建立方法同时测定了三七皂苷R₁，人参皂苷Rg₁、Re、Rb₁、Rd成分含量，方法学考察结果良好；灰色关联法可以区分出道地产区样品、合格样品和不合格样品。结论 所建立的HPLC测定方法简单准确、稳定快速、分离效果好，可用于三七皂苷R₁及人参皂苷Rg₁、Re、Rb₁、Rd的含量测定；灰色关联法可用于多指标成分中药质量评价。

Objective To establish a method for determination of five saponins in Panax notginseng by HPLC and comprehensively evaluate the quality of it by using grey correlation analysis. Methods The content of notoginsenoside R₁ and ginsenoside Rg₁, Re, Rb₁, Rd in the different origins and commercial grades of P. notginseng was simultaneously determined by HPLC, and the entire quality evaluation model was established by grey correlation analysis. Results The established method was applied to quantify five major bioactive components in P. notginseng simultaneously with satisfactory results. Gray correlation method can distinguish the samples from genuine producing areas, qualified samples and unqualified samples, and provide reference for quality evaluation of P. notoginseng and quality evaluation of multi-index components of Chinese materia medica. Conclusion This HPLC method was simple, accurate, stable and rapid with better separation effect, which was suitable for determination of notoginsenoside R₁ and ginsenosides Rg₁, Re, Rb₁, Rd; The grey recognition analysis was suitable for the comprehensive quality evaluation of multi-component samples of Chinese materia medica.

R286.2

北京市技术开发类横向课题（2017110031015577）