目的 探讨松萝烟酰胺对胃癌SGC-7901细胞的增殖抑制作用及机制。方法 体外培养SGC-7901细胞，分为对照组和不同浓度的松萝烟酰胺实验组，显微镜观察各组细胞形态；MTT法测定SGC-7901细胞增殖；AnnexinV/PI双染和DAPI荧光染色检测细胞凋亡；流式细胞术检测细胞周期；细胞划痕实验检测细胞的迁移。结果 松萝烟酰胺处理SGC-7901细胞后，细胞发生皱缩、变形，贴壁细胞脱落；MTT结果显示，松萝烟酰胺对SGC-7901增殖抑制作用呈明显的浓度依赖性和时间依赖性；AnnexinV/PI双染结果显示，松萝烟酰胺使SGC-7901细胞的晚期凋亡率增加，且DAPI染色可观察到明显的细胞凋亡的核浓缩、核碎裂；流式结果显示，松萝烟酰胺使SGC-7901细胞周期停滞在S期；划痕实验显示，随着时间的延长、浓度的增加，松萝烟酰胺使SGC-7901细胞迁移率下降越明显。结论 松萝烟酰胺对SGC-7901细胞具有增殖抑制作用，其作用机制可能是通过诱导细胞凋亡、阻滞细胞于S期，从而抑制细胞的迁移。

Objective To investigate the inhibitory effect of usnicoyinamide on the proliferation of gastric cancer cell line SGC-7901 and its mechanism. Methods SGC-7901 cells were cultured in vitro and divided into two groups:control group and experimental group with different concentrations of usnicoyinamide. The morphology of each group of cells was observed by a microscope; Proliferation of SGC-7901 cells was measured by MTT assay; The mechanism of apoptosis was studied by AnnexinV/PI double staining and DAPI fluorescence staining; Flow cytometry was used to detect the effect of usnicoyinamide on the cell cycle; Effect of usnicoyinamide on invasion and migration of SGC-7901 cells was detected by cell scratch test. Results After SGC-7901 cells were treated with usnicoyinamide, the cells were wrinkled, deformed and adherent cells fell off; The results of MTT showed that the inhibition of the proliferation of SGC-7901 cells was a significant dose-effect relationship and time-dependent; The results of AnnexinV/PI double staining showed that nicotine increased the late apoptosis rate of SGC-7901 cells, and DAPI staining showed obvious nuclear concentration and nuclear fragmentation of apoptosis. The results of flow cytometry showed that the cell cycle of SGC-7901 cells stagnated in S phase; Scratch test showed that the mobility of SGC-7901 cells was decreased more obviously with the prolongation of time and the increase of concentration. Conclusion Usnicoyinamide can inhibit the proliferation of gastric cancer cell line SGC-7901, and its mechanism may be achieved by inducing late apoptosis, inducing S phase cell arrest and inhibiting the invasion and migration of SGC-7901 cells.

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国家自然科学基金资助项目（81760538）；云南省科技厅科技计划项目（2018FE001）；昆明医科大学重大成果培育项目（CGPY201603）