[关键词]
[摘要]
目的 研究瑶药童子益母草与益母草多指标成分含量差异,并结合含量对品质进行评价。方法 采用高效液相色谱法测定瑶药童子益母草与益母草中绿原酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷5种化学成分含量,利用SPSS 25.0软件进行成对样品t检验及单因素方差分析推断各样品间含量差异,主成分分析评价药材品质。结果 童子益母草与益母草成对样品t检验结果表明,绿原酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷5种化学成分中芦丁含量呈显著性差异,其余成分无统计学差异;单因素方差分析结果表明,不同产地童子益母草和益母草药材中5种化学成分均存在极显著性差异(P<0.01);主成分分析结果显示,益母草得分较高的产地为南宁和玉林,上思县得分最低。结论 瑶药童子益母草与益母草含量存在显著差异,南宁和玉林的药材质量较佳,为2种益母草及不同产地益母草间含量的比较提供了数据支持。
[Key word]
[Abstract]
Objective To study the content difference of multi-index components between Yao medicine Young Leonurus heterophyllus (YLH) and Leonurus japonicus, and evaluate the quality with the content. Methods A high performance liquid chromatography (HPLC) method was established for the simultaneous content determination of five chemical constituents of chlorogenic acid, leonurine hydrochloride, rutin, hyperin and isoquercitrin in Yao medicine YLH and Leonurus japonicus. SPSS 25.0 statistical software was used to conduct paired samples t-test and one-way ANOVA to infer the content differences among the samples. Principal component analysis (PCA) was used to evaluate the quality of medicinal materials. Results T-test results of paired samples of Yao medicine YLH and Leonurus japonicus showed that there was significant difference in rutin content among the five chemical constituents, with no significant difference in other constituents. The results of one-way ANOVA analysis showed that there were significant differences in five chemical constituents of Yao medicine YLH and Leonurus japonicus from different habitats (P<0.01). The results of principal component analysis showed that the higher scores of Leonurus japonicus from different habitats were from Nanning and Yulin, with the lowest in Shangsi County. Conclusion There is a significant difference between the content of Yao medicine YLH and Leonurus japonicus. The quality of medicinal materials from Nanning and Yulin is better. The results provide data support for the comparison of the content between two kinds of Leonurus heterophyllus and Leonurus japonicus from different habitats.
[中图分类号]
R286.2
[基金项目]
广西研究生教育创新计划资助项目(YCSW2018176);广西壮瑶医药与医养结合人才小高地(厅发[2017]44号)