目的 基于细胞代谢组学的方法研究水栀子Gardenia jasminoside及其主要有效成分京尼平苷对RBL-2H3细胞脱颗粒模型的干预作用。方法 利用UPLC-QTOF-MS高分辨质谱检测RBL-2H3细胞的代谢轮廓改变，并应用主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）筛选出引起这种差异的代谢标志物；同时应用MEV软件对差异标志物水平的变化进行聚类分析和热图绘制。结果 分别得到与水栀子和京尼平苷作用相关的代谢标志物54和46个，其中共有标志物31个，且在2个给药组中变化趋势相同。由共有代谢标志物富集得到了受干扰的5条代谢通路：甘氨酸、天冬氨酸、谷氨酸代谢，谷胱甘肽代谢，组胺代谢，能量代谢，烟酰胺代谢。结论 水栀子和京尼平苷通过调节组胺代谢、氧化应激和能量代谢而抑制RBL-2H3细胞脱颗粒过程，且京尼平苷是水栀子发挥作用的主要药效物质基础之一。

Objective To study the intervention effect of Gardenia jasminoside var. radicans and its main effective component of geniposide on the degranulation model of RBL-2H3 cells based on metabolomics. Methods The changed metabolite profile of RBL-2H3 cells was detected by UPLC-QTOF-MS; PCA (principal component analysis) and OPLS-DA (orthogonal partial least squares discriminant analysis) in SIMCA software were used to select the potential biomarkers. Meanwhile, the clustering and heat map analysis for those potential biomarker levels were carried out by MEV software. Results A total of 54 and 46 relevant biomarkers of G. jasminoside var. radicans and geniposide were selected, of which 31 biomarkers enriched in five disturbed metabolite pathways, including glycine, aspartic acid and glutamate metabolism, glutathione metabolism, histamine metabolism, energy metabolism, and nicotinamide metabolism pathways. Conclusion G. jasminoside var. radicans and geniposide exerts the inhibitory effect on the degranulation model of RBL-2H3 cells by regulating histamine metabolism, oxidative stress and energy metabolism, and geniposide was one of the main efficacious substance basis of G. jasminoside var. radicans.

R285.5

中央引导地方科技发展专项：河南道地大宗药材种质评价及集约化种植与示范（豫财科[2016]149号）；河南省高层次人才特殊支持计划“中原千人计划”-中原领军人才（ZYQR201810080）；2018年度河南省重大科技专项（国际合作）：水栀子抗动脉粥样硬化药效物质基础研究（182102410080）