目的 优选超临界CO₂提取厚朴有效成分的工艺并探讨厚朴超临界CO₂提取物的抗氧化活性。方法 采用HPLC法测定厚朴超临界CO₂提取物中厚朴酚与和厚朴酚的含量，正交试验优选厚朴超临界CO₂提取工艺，MTT法检测提取物抗氧化活性。结果 厚朴酚优化工艺为萃取压力25 MPa，萃取温度55℃，CO₂用量30 kg；和厚朴酚最佳提取工艺压力15 MPa、萃取温度50℃、CO₂用量25 kg。厚朴超临界CO₂提取物具有抗氧化活性，且分离参数不同，抗氧化活性有显著差异。结论 在所优选的提取工艺条件下，厚朴酚、和厚朴酚的提取效率较高，重复性较好，工艺稳定可行，提取物具有良好的抗氧化活性。

Objective To optimize the supercritical CO₂ extraction process of active ingredients from Magnoliae Officinalis Cortex (MOC) and explore the antioxidant activity of the extracts. Methods The content of magnolol and honokiol of the supercritical CO₂ extracts of MOC was determined by HPLC, and the extraction process was optimized by orthogonal experiment. The antioxidant activities of the extracts were determined by MTT. Results The optimum extraction pressure of magnolol was 25 MPa, the extraction temperature was 55℃, the amount of CO₂ was 30 kg, and the optimum extraction parameters mentioned above of honokiol were 15 MPa, 50℃, and 25 kg, respectively. Conclusion Under the optimum extraction conditions, magnolol and honokiol have high extraction efficiency, good repeatability, stability and feasibility, and the extract have good antioxidant activity.

R284.2

湖南省重点研发计划项目（2018NK2041）；湖南省科技厅重点实验室建设项目（2016TP1017）；湖南省教育厅优秀青年基金项目（2015B172）；湖南省“十二五”省级药学重点学科（1007）开放基金项目；湖南中医药大学化学工程与技术一流学科建设项目