目的 研究小驳骨Gendarussa vulgaris地上部位的萜类化学成分。方法 采用硅胶、Sephadex LH-20、反相ODS、大孔吸附树脂AB-8等柱色谱方法以及半制备HPLC法进行分离纯化，根据化合物理化性质和波谱数据进行结构鉴定。结果 从小驳骨药材95%乙醇总提取物中共分离得到10个萜类化合物，分别鉴定为驳骨萜A（1）、4，4，14α-三甲基孕甾-8-烯-3β，20α-二醇（2）、华木酮（3）、过氧化麦角甾醇（4）、熊果酸（5）、齐墩果酸（6）、3β-羟基-11α，12α-环氧齐墩果-28，13β-内酯（7）、当药苷（8）、马钱子苷（9）、脱水莫诺苷元（10）。结论 化合物1为未见文献报道的新化合物，命名为驳骨萜A；化合物2为新天然产物，并首次对其氢谱和碳谱数据进行归属；化合物3～5、7～10均为首次从该属植物中分离得到。

Objective To isolate and identify the terpenoids from the aerial parts of Gendarussa vulgaris. Methods The 95% EtOH extract of the aerial parts of G. vulgaris were isolated and purified by silica gel, Sephadex LH-20, reversed-phase ODS, macroporous adsorption resin AB-8 and semi-preparative high performance liquid chromatography. The compound structures were identified by physicochemical properties and spectroscopic data. Results Ten terpenoids were identified as gvterpennoid A (1), 4,4,14α-trimethylpregn-8-en-3β,20α-diol (2), betulone (3), ergosterol endoperoxide (4), ursolic acid (5), oleanolic acid (6), 3β-hydroxyl-11α,12α-epoxy olean-28,13β-lactone (7), sweroside (8), loganin (9), and dehydromorroniaglycone (10). Conclusion Compound 1 is a new triterpene, named gvterpennoid A. Compound 2 is a new natural product, and its ¹³C- and ¹H-NMR chemical shifts were first completely assigned on the basis of 1D and 2D NMR spectroscopic evidence. Compounds 3-5, 7-10 are isolated from Gendarussa genus for the first time.

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河南省科技攻关项目（192102310438）；河南中医药大学省属高校基本科研业务专项资金项目（2014KYYWF-QN26）；河南中医药大学科技创新人才支持计划（2016XCXRC01）；河南省张仲景方药与免疫调节重点实验室开放课题资助项目（kfkt201701）